Characterization of DNA Repair Deficient Strains of
Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

J 2014

Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

PLECENIKOVA, A., M. SLANINOVA a Karel ŘÍHA

Základní údaje

Originální název

Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

Autoři

PLECENIKOVA, A. (703 Slovensko), M. SLANINOVA (703 Slovensko) a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

Plos one, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2014, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.234

Kód RIV

RIV/00216224:14740/14:00079173

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1371/journal.pone.0105482

UT WoS

000341106100073

Klíčová slova anglicky

HOMOLOGOUS RECOMBINATION; NUCLEAR TRANSFORMATION; HUMAN CTIP; ARG7 GENE; ARABIDOPSIS; PLANTS; GENOME; DAMAGE; MECHANISMS; RESPONSES

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 2. 2015 11:17, Martina Prášilová

Anotace

V originále

While the mechanisms governing DNA damage response and repair are fundamentally conserved, cross-kingdom comparisons indicate that they differ in many aspects due to differences in life-styles and developmental strategies. In photosynthetic organisms these differences have not been fully explored because gene-discovery approaches are mainly based on homology searches with known DDR/DNA repair proteins. Here we performed a forward genetic screen in the green algae Chlamydomonas reinhardtii to identify genes deficient in DDR/DNA repair. We isolated five insertional mutants that were sensitive to various genotoxic insults and two of them exhibited altered efficiency of transgene integration. To identify genomic regions disrupted in these mutants, we established a novel adaptor-ligation strategy for the efficient recovery of the insertion flanking sites. Four mutants harbored deletions that involved known DNA repair factors, DNA Pol zeta, DNA Pol theta, SAE2/COM1, and two neighbouring genes encoding ERCC1 and RAD17. Deletion in the last mutant spanned two Chlamydomonas-specific genes with unknown function, demonstrating the utility of this approach for discovering novel factors involved in genome maintenance.

Citovat

PLECENIKOVA, A., M. SLANINOVA a Karel ŘÍHA. Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis. Plos one. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2014, roč. 9, č. 8, s. "nestránkováno", 9 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0105482.

@article{1227576,
   author = {Plecenikova, A. and Slaninova, M. and Říha, Karel},
   article_location = {SAN FRANCISCO},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0105482},
   keywords = {HOMOLOGOUS RECOMBINATION; NUCLEAR TRANSFORMATION; HUMAN CTIP; ARG7 GENE; ARABIDOPSIS; PLANTS; GENOME; DAMAGE; MECHANISMS; RESPONSES},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis},
   url = {http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1227576
AU  - Plecenikova, A. - Slaninova, M. - Říha, Karel
PY  - 2014
TI  - Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis
JF  - Plos one
VL  - 9
IS  - 8
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - HOMOLOGOUS RECOMBINATION
KW  - NUCLEAR TRANSFORMATION
KW  - HUMAN CTIP
KW  - ARG7 GENE
KW  - ARABIDOPSIS
KW  - PLANTS
KW  - GENOME
KW  - DAMAGE
KW  - MECHANISMS
KW  - RESPONSES
UR  - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
L2  - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
N2  - While the mechanisms governing DNA damage response and repair are fundamentally conserved, cross-kingdom comparisons indicate that they differ in many aspects due to differences in life-styles and developmental strategies. In photosynthetic organisms these differences have not been fully explored because gene-discovery approaches are mainly based on homology searches with known DDR/DNA repair proteins. Here we performed a forward genetic screen in the green algae Chlamydomonas reinhardtii to identify genes deficient in DDR/DNA repair. We isolated five insertional mutants that were sensitive to various genotoxic insults and two of them exhibited altered efficiency of transgene integration. To identify genomic regions disrupted in these mutants, we established a novel adaptor-ligation strategy for the efficient recovery of the insertion flanking sites. Four mutants harbored deletions that involved known DNA repair factors, DNA Pol zeta, DNA Pol theta, SAE2/COM1, and two neighbouring genes encoding ERCC1 and RAD17. Deletion in the last mutant spanned two Chlamydomonas-specific genes with unknown function, demonstrating the utility of this approach for discovering novel factors involved in genome maintenance.
ER  -

PLECENIKOVA, A., M. SLANINOVA a Karel ŘÍHA. Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis. \textit{Plos one}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2014, roč.~9, č.~8, s.~''nestránkováno'', 9 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0105482.

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