| DERBOVEN, E., H. EKKER, B. KUSENDA, Petra BULÁNKOVÁ a Karel ŘÍHA. Role of STN1 and DNA Polymerase alpha in Telomere Stability and Genome-Wide Replication in Arabidopsis. PLoS Genetics. San Francisco, California, United States: Public Library Science, 2014, roč. 10, č. 10, s. "nestránkováno", 13 s. ISSN 1553-7390. Dostupné z: https://dx.doi.org/10.1371/journal.pgen.1004682. |
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@article{1227577,
author = {Derboven, E. and Ekker, H. and Kusenda, B. and Bulánková, Petra and Říha, Karel},
article_location = {San Francisco, California, United States},
article_number = {10},
doi = {http://dx.doi.org/10.1371/journal.pgen.1004682},
keywords = {SINGLE-STRANDED-DNA; CATALYTIC SUBUNIT; HUMAN CST; FILL-IN; CHECKPOINT ACTIVATION; DUPLEX REPLICATION; LENGTH REGULATION; CHROMOSOME ENDS; PROTEIN COMPLEX; MUTANTS},
language = {eng},
issn = {1553-7390},
journal = {PLoS Genetics},
title = {Role of STN1 and DNA Polymerase alpha in Telomere Stability and Genome-Wide Replication in Arabidopsis},
url = {http://www.plosgenetics.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pgen.1004682&representation=PDF},
volume = {10},
year = {2014}
}
TY - JOUR
ID - 1227577
AU - Derboven, E. - Ekker, H. - Kusenda, B. - Bulánková, Petra - Říha, Karel
PY - 2014
TI - Role of STN1 and DNA Polymerase alpha in Telomere Stability and Genome-Wide Replication in Arabidopsis
JF - PLoS Genetics
VL - 10
IS - 10
SP - "nestránkováno"
EP - "nestránkováno"
PB - Public Library Science
SN - 15537390
KW - SINGLE-STRANDED-DNA
KW - CATALYTIC SUBUNIT
KW - HUMAN CST
KW - FILL-IN
KW - CHECKPOINT ACTIVATION
KW - DUPLEX REPLICATION
KW - LENGTH REGULATION
KW - CHROMOSOME ENDS
KW - PROTEIN COMPLEX
KW - MUTANTS
UR - http://www.plosgenetics.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pgen.1004682&representation=PDF
L2 - http://www.plosgenetics.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pgen.1004682&representation=PDF
N2 - The CST (Cdc13/CTC1-STN1-TEN1) complex was proposed to have evolved kingdom specific roles in telomere capping and replication. To shed light on its evolutionary conserved function, we examined the effect of STN1 dysfunction on telomere structure in plants. STN1 inactivation in Arabidopsis leads to a progressive loss of telomeric DNA and the onset of telomeric defects depends on the initial telomere size. While EXO1 aggravates defects associated with STN1 dysfunction, it does not contribute to the formation of long G-overhangs. Instead, these G-overhangs arise, at least partially, from telomerase-mediated telomere extension indicating a deficiency in C-strand fill-in synthesis. Analysis of hypomorphic DNA polymerase a mutants revealed that the impaired function of a general replication factor mimics the telomeric defects associated with CST dysfunction. Furthermore, we show that STN1-deficiency hinders re-replication of heterochromatic regions to a similar extent as polymerase alpha mutations. This comparative analysis of stn1 and pol alpha mutants suggests that STN1 plays a genome-wide role in DNA replication and that chromosome-end deprotection in stn1 mutants may represent a manifestation of aberrant replication through telomeres.
ER -
DERBOVEN, E., H. EKKER, B. KUSENDA, Petra BULÁNKOVÁ a Karel ŘÍHA. Role of STN1 and DNA Polymerase alpha in Telomere Stability and Genome-Wide Replication in Arabidopsis. \textit{PLoS Genetics}. San Francisco, California, United States: Public Library Science, 2014, roč.~10, č.~10, s.~''nestránkováno'', 13 s. ISSN~1553-7390. Dostupné z: https://dx.doi.org/10.1371/journal.pgen.1004682.
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