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@article{1227589,
author = {Plevka, Pavel and Lim, P.Y. and Perera, R. and Cardosa, J. and Suksatu, A. and Kuhn, R.J. and Rossmann, M.G.},
article_location = {WASHINGTON},
article_number = {6},
doi = {http://dx.doi.org/10.1073/pnas.1320624111},
keywords = {DECAY-ACCELERATING FACTOR; CELL ENTRY; CRYSTAL-STRUCTURE; POLIOVIRUS; RECEPTOR; INTERMEDIATE; PICORNAVIRUS; PARTICLE; VIRUS; TRANSITIONS},
language = {eng},
issn = {0027-8424},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
title = {Neutralizing antibodies can initiate genome release from human enterovirus 71},
url = {http://www.pnas.org/content/111/6/2134.full},
volume = {111},
year = {2014}
}
TY - JOUR
ID - 1227589
AU - Plevka, Pavel - Lim, P.Y. - Perera, R. - Cardosa, J. - Suksatu, A. - Kuhn, R.J. - Rossmann, M.G.
PY - 2014
TI - Neutralizing antibodies can initiate genome release from human enterovirus 71
JF - Proceedings of the National Academy of Sciences of the United States of America
VL - 111
IS - 6
SP - 2134-2139
EP - 2134-2139
PB - NATL ACAD SCIENCES
SN - 00278424
KW - DECAY-ACCELERATING FACTOR
KW - CELL ENTRY
KW - CRYSTAL-STRUCTURE
KW - POLIOVIRUS
KW - RECEPTOR
KW - INTERMEDIATE
KW - PICORNAVIRUS
KW - PARTICLE
KW - VIRUS
KW - TRANSITIONS
UR - http://www.pnas.org/content/111/6/2134.full
L2 - http://www.pnas.org/content/111/6/2134.full
N2 - Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 degrees C and 37 degrees C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.
ER -
PLEVKA, Pavel, P.Y. LIM, R. PERERA, J. CARDOSA, A. SUKSATU, R.J. KUHN and M.G. ROSSMANN. Neutralizing antibodies can initiate genome release from human enterovirus 71. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: NATL ACAD SCIENCES, 2014, vol.~111, No~6, p.~2134-2139. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1320624111.
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