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@article{1227589, author = {Plevka, Pavel and Lim, P.Y. and Perera, R. and Cardosa, J. and Suksatu, A. and Kuhn, R.J. and Rossmann, M.G.}, article_location = {WASHINGTON}, article_number = {6}, doi = {http://dx.doi.org/10.1073/pnas.1320624111}, keywords = {DECAY-ACCELERATING FACTOR; CELL ENTRY; CRYSTAL-STRUCTURE; POLIOVIRUS; RECEPTOR; INTERMEDIATE; PICORNAVIRUS; PARTICLE; VIRUS; TRANSITIONS}, language = {eng}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, title = {Neutralizing antibodies can initiate genome release from human enterovirus 71}, url = {http://www.pnas.org/content/111/6/2134.full}, volume = {111}, year = {2014} }
TY - JOUR ID - 1227589 AU - Plevka, Pavel - Lim, P.Y. - Perera, R. - Cardosa, J. - Suksatu, A. - Kuhn, R.J. - Rossmann, M.G. PY - 2014 TI - Neutralizing antibodies can initiate genome release from human enterovirus 71 JF - Proceedings of the National Academy of Sciences of the United States of America VL - 111 IS - 6 SP - 2134-2139 EP - 2134-2139 PB - NATL ACAD SCIENCES SN - 00278424 KW - DECAY-ACCELERATING FACTOR KW - CELL ENTRY KW - CRYSTAL-STRUCTURE KW - POLIOVIRUS KW - RECEPTOR KW - INTERMEDIATE KW - PICORNAVIRUS KW - PARTICLE KW - VIRUS KW - TRANSITIONS UR - http://www.pnas.org/content/111/6/2134.full L2 - http://www.pnas.org/content/111/6/2134.full N2 - Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 degrees C and 37 degrees C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles. ER -
PLEVKA, Pavel, P.Y. LIM, R. PERERA, J. CARDOSA, A. SUKSATU, R.J. KUHN and M.G. ROSSMANN. Neutralizing antibodies can initiate genome release from human enterovirus 71. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: NATL ACAD SCIENCES, 2014, vol.~111, No~6, p.~2134-2139. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1320624111.
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