Další formáty:
BibTeX
LaTeX
RIS
@article{1227612,
author = {Simonova, Anna and Bálintová, Jarmila and Pohl, Radek and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
article_location = {Weinheim},
article_number = {12},
doi = {http://dx.doi.org/10.1002/cplu.201402194},
keywords = {DNA; electrochemistry; nucleotides; polymerase chain reactions; redox labeling},
language = {eng},
issn = {2192-6506},
journal = {CHEMPLUSCHEM},
title = {Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA},
url = {http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full},
volume = {79},
year = {2014}
}
TY - JOUR
ID - 1227612
AU - Simonova, Anna - Bálintová, Jarmila - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY - 2014
TI - Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA
JF - CHEMPLUSCHEM
VL - 79
IS - 12
SP - 1703-1712
EP - 1703-1712
PB - Wiley - Verlag Chemie
SN - 21926506
KW - DNA
KW - electrochemistry
KW - nucleotides
KW - polymerase chain reactions
KW - redox labeling
UR - http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full
L2 - http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full
N2 - 4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA.
ER -
SIMONOVA, Anna, Jarmila BÁLINTOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA a Michal HOCEK. Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA. \textit{CHEMPLUSCHEM}. Weinheim: Wiley - Verlag Chemie, 2014, roč.~79, č.~12, s.~1703-1712. ISSN~2192-6506. Dostupné z: https://dx.doi.org/10.1002/cplu.201402194.
|
