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@article{1229044,
author = {Pescheck, michael and Durr, Constanze and Bláha, Luděk and Sell, Dieter},
article_location = {DORDRECHT},
article_number = {8},
doi = {http://dx.doi.org/10.1007/s10800-014-0707-x},
keywords = {Bioelectrochemistry; Mediator; Cytotoxicity; IC50; Cytotoxicity test; Toxicological risk; Bioactivity sensor},
language = {eng},
issn = {0021-891X},
journal = {JOURNAL OF APPLIED ELECTROCHEMISTRY},
title = {Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method},
url = {http://link.springer.com/article/10.1007%2Fs10800-014-0707-x},
volume = {44},
year = {2014}
}
TY - JOUR
ID - 1229044
AU - Pescheck, michael - Durr, Constanze - Bláha, Luděk - Sell, Dieter
PY - 2014
TI - Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method
JF - JOURNAL OF APPLIED ELECTROCHEMISTRY
VL - 44
IS - 8
SP - 935-943
EP - 935-943
PB - SPRINGER
SN - 0021891X
KW - Bioelectrochemistry
KW - Mediator
KW - Cytotoxicity
KW - IC50
KW - Cytotoxicity test
KW - Toxicological risk
KW - Bioactivity sensor
UR - http://link.springer.com/article/10.1007%2Fs10800-014-0707-x
L2 - http://link.springer.com/article/10.1007%2Fs10800-014-0707-x
N2 - The proliferation of pharmaceuticals and chemicals and the increasing manufacture of medical goods have made an assessment of their potential toxicological risks to man and the environment indispensable. Hence, a wide range of tests has been developed in order to generate data on the harmful effects of chemicals and pharmaceuticals on organisms. Initially, toxicological data were commonly collected from tests involving animals; however, on account of ethical objections, they are gradually being replaced by in vitro cytotoxicity tests. In this work, a new in vitro screening method for the determination of cytotoxicity, based on a novel electrochemical bioactivity sensor system, was implemented. The evaluation was based on current-time curves of a potentiostatic measurement proportional to the reduction of mediator molecules by mammalian cells. Depending on the number of reduced mediator molecules used by the cells, a current signal is produced, which provides information about the viability of living cells. By adding a toxic test substance to the cell, a reduction in the current signal can be observed, depending on the cytotoxicity of the substance. It is possible to quickly create a specific cytotoxic curve and to determine the corresponding inhibitory concentrations (IC50 value). First tests were performed on three mammalian cell lines and eight model compounds using this electrochemical measurement system. The IC50 values obtained corresponded well with the toxicity determined with an established reference cytotoxicity assay (MTT test) and with data reported in the literature.
ER -
PESCHECK, michael, Constanze DURR, Luděk BLÁHA a Dieter SELL. Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method. \textit{JOURNAL OF APPLIED ELECTROCHEMISTRY}. DORDRECHT: SPRINGER, 2014, roč.~44, č.~8, s.~935-943. ISSN~0021-891X. Dostupné z: https://dx.doi.org/10.1007/s10800-014-0707-x.
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