Effects of clofibric acid alone and in combination with 17
beta-estradiol on mRNA abundance in primary hepatocytes
isolated from rainbow trout

J 2014

Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout

SOVADINOVÁ, Iva, A. LIEDTKE and K. SCHIRMER

Basic information

Original name

Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout

Authors

SOVADINOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution), A. LIEDTKE (276 Germany) and K. SCHIRMER (276 Germany)

Edition

Toxicology in vitro, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2014, 0887-2333

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.903

RIV identification code

RIV/00216224:14310/14:00079361

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.tiv.2014.05.002

UT WoS

000339599300003

Keywords in English

Clofibric acid; Primary fish hepatocytes; Rainbow trout; Vitellogenin; Endocrine disruption

Abstract

V originále

Clofibric acid (CA) is the active substance of lipid lowering drugs. It is resistant to degradation, polar in nature, and has been found ubiquitously in the aquatic environment. Though CA is classified as a peroxisomal proliferator in rodents and is considered as a potential endocrine disruptor, little information exists on the effects of CA in aquatic organisms, such as fish. In the present study, we examined the mRNA levels of peroxisome proliferator- and estrogen-sensitive genes on the exposure of primary rainbow trout (Oncorhynchus mykiss) hepatocytes to CA alone and in combination with the natural female sex hormone, 17 beta-estradiol (E2). Our results demonstrate that rainbow trout hepatocytes are relatively refractory to the effects of CA on the PPAR signaling pathway and lipid metabolism. Moreover, CA did not show recognizable estrogenic activity, but after the induction of vitellogenesis by E2, CA significantly reduced vitellogenin (VTG) mRNA abundance. Apparently, the indirect repression of VTG transcription, independent of estrogen receptors, occurred. The mechanism is not yet clearly understood but may involve disruption of the stabilization of VTG mRNA known to be induced by E2. (C) 2014 Elsevier Ltd. All rights reserved.

Citovat

SOVADINOVÁ, Iva, A. LIEDTKE and K. SCHIRMER. Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout. Toxicology in vitro. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2014, vol. 28, No 6, p. 1106-1116. ISSN 0887-2333. Available from: https://dx.doi.org/10.1016/j.tiv.2014.05.002.

@article{1229052,
   author = {Sovadinová, Iva and Liedtke, A. and Schirmer, K.},
   article_location = {OXFORD},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.tiv.2014.05.002},
   keywords = {Clofibric acid; Primary fish hepatocytes; Rainbow trout; Vitellogenin; Endocrine disruption},
   language = {eng},
   issn = {0887-2333},
   journal = {Toxicology in vitro},
   title = {Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout},
   url = {http://www.sciencedirect.com/science/article/pii/S0887233314000824},
   volume = {28},
   year = {2014}
}

TY  - JOUR
ID  - 1229052
AU  - Sovadinová, Iva - Liedtke, A. - Schirmer, K.
PY  - 2014
TI  - Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout
JF  - Toxicology in vitro
VL  - 28
IS  - 6
SP  - 1106-1116
EP  - 1106-1116
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 08872333
KW  - Clofibric acid
KW  - Primary fish hepatocytes
KW  - Rainbow trout
KW  - Vitellogenin
KW  - Endocrine disruption
UR  - http://www.sciencedirect.com/science/article/pii/S0887233314000824
L2  - http://www.sciencedirect.com/science/article/pii/S0887233314000824
N2  - Clofibric acid (CA) is the active substance of lipid lowering drugs. It is resistant to degradation, polar in nature, and has been found ubiquitously in the aquatic environment. Though CA is classified as a peroxisomal proliferator in rodents and is considered as a potential endocrine disruptor, little information exists on the effects of CA in aquatic organisms, such as fish. In the present study, we examined the mRNA levels of peroxisome proliferator- and estrogen-sensitive genes on the exposure of primary rainbow trout (Oncorhynchus mykiss) hepatocytes to CA alone and in combination with the natural female sex hormone, 17 beta-estradiol (E2). Our results demonstrate that rainbow trout hepatocytes are relatively refractory to the effects of CA on the PPAR signaling pathway and lipid metabolism. Moreover, CA did not show recognizable estrogenic activity, but after the induction of vitellogenesis by E2, CA significantly reduced vitellogenin (VTG) mRNA abundance. Apparently, the indirect repression of VTG transcription, independent of estrogen receptors, occurred. The mechanism is not yet clearly understood but may involve disruption of the stabilization of VTG mRNA known to be induced by E2. (C) 2014 Elsevier Ltd. All rights reserved.
ER  -

SOVADINOVÁ, Iva, A. LIEDTKE and K. SCHIRMER. Effects of clofibric acid alone and in combination with 17 beta-estradiol on mRNA abundance in primary hepatocytes isolated from rainbow trout. \textit{Toxicology in vitro}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2014, vol.~28, No~6, p.~1106-1116. ISSN~0887-2333. Available from: https://dx.doi.org/10.1016/j.tiv.2014.05.002.

Detailed Information on Publication Record