Endocrine, teratogenic and neurotoxic effects of cyanobacteria
detected by cellular in vitro and zebrafish embryos assays

J 2015

Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays

JONÁŠ, Adam, Stefan SCHOLZ, Eva FETTER, Eliška SYCHROVÁ, Kateřina NOVÁKOVÁ et. al.

Základní údaje

Originální název

Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays

Autoři

JONÁŠ, Adam (203 Česká republika, domácí), Stefan SCHOLZ (276 Německo), Eva FETTER (276 Německo), Eliška SYCHROVÁ (203 Česká republika, domácí), Kateřina NOVÁKOVÁ (203 Česká republika, domácí), Julia ORTMANN (276 Německo), Martin BENÍŠEK (203 Česká republika, domácí), Ondřej ADAMOVSKÝ (203 Česká republika, domácí), John P. GIESY (124 Kanada) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Chemosphere, OXFORD (ENGLAND), Elsevier Science, 2015, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.698

Kód RIV

RIV/00216224:14310/15:00080689

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2014.07.074

UT WoS

000348003200044

Klíčová slova anglicky

Estrogenicity; Teratogenicity; Retinoid-like activity; Blue-green algae; Fish

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 4. 2016 10:03, Ing. Andrea Mikešková

Anotace

V originále

Cyanobacteria contain various types of bioactive compounds, which could cause adverse effects on organisms. They are released into surface waters during cyanobacterial blooms, but there is little information on their potential relevance for effects in vivo. In this study presence of bioactive compounds was characterized in cyanobacteria Microcystis aeruginosa (Chroococcales), Planktothrix agardhii (Oscillatoriales) and Aphanizomenon gracile (Nostocales) with selected in vitro assays. The in vivo relevance of detected bioactivities was analysed using transgenic zebrafish embryos tg(cypi9a1b-GFP). Teratogenic potency was assessed by analysis of developmental disorders and effects on functions of the neuromuscular system by video tracking of locomotion. Estrogenicity in vitro corresponded to 0.95-54.6 ng estradiol equivalent (g dry weight (dw))(-1). In zebrafish embryos, estrogenic effects could not be detected potentially because they were masked by high toxicity. There was no detectable (anti)androgenic/glucocorticoid activity in any sample. Retinoid-like activity was determined at 1-1.3 mu g all-trans-retinoic acid equivalent (g dw)-1. Corresponding to the retinoid-like activity A. gracile extract also caused teratogenic effects in zebrafish embryos. Furthermore, exposure to biomass extracts at 0.3 g dw L-1 caused increase of body length in embryos. There were minor effects on locomotion caused by 0.3 g dw L-1 M. aeruginosa and P. agardhii extracts. The traditionally measured cyanotoxins microcystins did not seem to play significant role in observed effects. This indicates importance of other cyanobacterial compounds at least towards some species or their developmental phases. More attention should be paid to activity of retinoids, estrogens and other bioactive substances in phytoplankton using in vitro and in vivo bioassays.

Návaznosti

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

Citovat

JONÁŠ, Adam, Stefan SCHOLZ, Eva FETTER, Eliška SYCHROVÁ, Kateřina NOVÁKOVÁ, Julia ORTMANN, Martin BENÍŠEK, Ondřej ADAMOVSKÝ, John P. GIESY a Klára HILSCHEROVÁ. Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays. Chemosphere. OXFORD (ENGLAND): Elsevier Science, 2015, roč. 120, FEB, s. 321-327. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2014.07.074.

@article{1229134,
   author = {Jonáš, Adam and Scholz, Stefan and Fetter, Eva and Sychrová, Eliška and Nováková, Kateřina and Ortmann, Julia and Beníšek, Martin and Adamovský, Ondřej and Giesy, John P. and Hilscherová, Klára},
   article_location = {OXFORD (ENGLAND)},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2014.07.074},
   keywords = {Estrogenicity; Teratogenicity; Retinoid-like activity; Blue-green algae; Fish},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays},
   url = {http://www.sciencedirect.com/science/article/pii/S0045653514009370},
   volume = {120},
   year = {2015}
}

TY  - JOUR
ID  - 1229134
AU  - Jonáš, Adam - Scholz, Stefan - Fetter, Eva - Sychrová, Eliška - Nováková, Kateřina - Ortmann, Julia - Beníšek, Martin - Adamovský, Ondřej - Giesy, John P. - Hilscherová, Klára
PY  - 2015
TI  - Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays
JF  - Chemosphere
VL  - 120
IS  - FEB
SP  - 321-327
EP  - 321-327
PB  - Elsevier Science
SN  - 00456535
KW  - Estrogenicity
KW  - Teratogenicity
KW  - Retinoid-like activity
KW  - Blue-green algae
KW  - Fish
UR  - http://www.sciencedirect.com/science/article/pii/S0045653514009370
L2  - http://www.sciencedirect.com/science/article/pii/S0045653514009370
N2  - Cyanobacteria contain various types of bioactive compounds, which could cause adverse effects on organisms. They are released into surface waters during cyanobacterial blooms, but there is little information on their potential relevance for effects in vivo. In this study presence of bioactive compounds was characterized in cyanobacteria Microcystis aeruginosa (Chroococcales), Planktothrix agardhii (Oscillatoriales) and Aphanizomenon gracile (Nostocales) with selected in vitro assays. The in vivo relevance of detected bioactivities was analysed using transgenic zebrafish embryos tg(cypi9a1b-GFP). Teratogenic potency was assessed by analysis of developmental disorders and effects on functions of the neuromuscular system by video tracking of locomotion. Estrogenicity in vitro corresponded to 0.95-54.6 ng estradiol equivalent (g dry weight (dw))(-1). In zebrafish embryos, estrogenic effects could not be detected potentially because they were masked by high toxicity. There was no detectable (anti)androgenic/glucocorticoid activity in any sample. Retinoid-like activity was determined at 1-1.3 mu g all-trans-retinoic acid equivalent (g dw)-1. Corresponding to the retinoid-like activity A. gracile extract also caused teratogenic effects in zebrafish embryos. Furthermore, exposure to biomass extracts at 0.3 g dw L-1 caused increase of body length in embryos. There were minor effects on locomotion caused by 0.3 g dw L-1 M. aeruginosa and P. agardhii extracts. The traditionally measured cyanotoxins microcystins did not seem to play significant role in observed effects. This indicates importance of other cyanobacterial compounds at least towards some species or their developmental phases. More attention should be paid to activity of retinoids, estrogens and other bioactive substances in phytoplankton using in vitro and in vivo bioassays.
ER  -

JONÁŠ, Adam, Stefan SCHOLZ, Eva FETTER, Eliška SYCHROVÁ, Kateřina NOVÁKOVÁ, Julia ORTMANN, Martin BENÍŠEK, Ondřej ADAMOVSKÝ, John P. GIESY a Klára HILSCHEROVÁ. Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays. \textit{Chemosphere}. OXFORD (ENGLAND): Elsevier Science, 2015, roč.~120, FEB, s.~321-327. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2014.07.074.

Podrobný výpis o publikaci