Characterization of Enterococcus species occuring on
terrestrial vegetation

k 2014

Characterization of Enterococcus species occuring on terrestrial vegetation

ŠVEC, Pavel, Lucie ZÁTOPKOVÁ, Hana BRYNDOVÁ a Ivo SEDLÁČEK

Základní údaje

Originální název

Characterization of Enterococcus species occuring on terrestrial vegetation

Autoři

ŠVEC, Pavel, Lucie ZÁTOPKOVÁ, Hana BRYNDOVÁ a Ivo SEDLÁČEK

Vydání

International Union of Microbiological Societies Congresses (IUMS 2014), 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Enterococcus; terrestrial vegetation; taxonomy; ecology

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2015 11:34, doc. RNDr. Pavel Švec, Ph.D.

Anotace

V originále

A group of 173 presumptive plant-associated enterococcal strains was isolated during the vegetation periods in 2009 and 2013 in the frame of two studies dealing with the investigation of Enterococcus spp. populations occurring on plants. Individual strains of Gram-positive cocci were isolated on Kanamycin esculin azide agar and characterized using rep-PCR fingerprinting with the (GTG)5 primer. Obtained fingerprints were compared with an in-house CCM reference database containing more that 7000 fingerprints representing multiple Gram-positive species including representatives of all hitherto described Enterococcus spp. In total, 102 isolates matched reference database entries and were assigned as Enterococcus faecalis (23 strains), Enterococcus haemoperoxidus (20), Enterococcus plantarum (14), Enterococcus casseliflavus (12), Enterococcus faecium (9), Enterococcus ureilyticus (8), Enterococcus moraviensis (6), Enterococcus rotai (6), Enterococcus mundtii (2), and Enterococcus durans (2). Remaining 71 strains were identified as members of other genera (e.g. Lactococcus, Leuconostoc) or were not clustered with any fingerprints included in the database. Overall results obtained in the frame of the present work revealed that plants are inhabited by diverse and taxonomically rich Enterococcus spp. populations. This work was supported by the project CZ.1.07/2.3.00/20.0183.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

ŠVEC, Pavel, Lucie ZÁTOPKOVÁ, Hana BRYNDOVÁ a Ivo SEDLÁČEK. Characterization of Enterococcus species occuring on terrestrial vegetation. In International Union of Microbiological Societies Congresses (IUMS 2014). 2014.

@proceedings{1229173,
   author = {Švec, Pavel and Zátopková, Lucie and Bryndová, Hana and Sedláček, Ivo},
   booktitle = {International Union of Microbiological Societies Congresses (IUMS 2014)},
   keywords = {Enterococcus; terrestrial vegetation; taxonomy; ecology},
   language = {eng},
   title = {Characterization of Enterococcus species occuring on terrestrial vegetation},
   year = {2014}
}

TY  - CONF
ID  - 1229173
AU  - Švec, Pavel - Zátopková, Lucie - Bryndová, Hana - Sedláček, Ivo
PY  - 2014
TI  - Characterization of Enterococcus species occuring on terrestrial vegetation
KW  - Enterococcus
KW  - terrestrial vegetation
KW  - taxonomy
KW  - ecology
N2  - A group of 173 presumptive plant-associated enterococcal strains was isolated during the vegetation periods in 2009 and 2013 in the frame of two studies dealing with the investigation of Enterococcus spp. populations occurring on plants. Individual strains of Gram-positive cocci were isolated on Kanamycin esculin azide agar and characterized using rep-PCR fingerprinting with the (GTG)5 primer. Obtained fingerprints were compared with an in-house CCM reference database containing more that 7000 fingerprints representing multiple Gram-positive species including representatives of all hitherto described Enterococcus spp. In total, 102 isolates matched reference database entries and were assigned as Enterococcus faecalis (23 strains), Enterococcus haemoperoxidus (20), Enterococcus plantarum (14), Enterococcus casseliflavus (12), Enterococcus faecium (9), Enterococcus ureilyticus (8), Enterococcus moraviensis (6), Enterococcus rotai (6), Enterococcus mundtii (2), and Enterococcus durans (2). Remaining 71 strains were identified as members of other genera (e.g. Lactococcus, Leuconostoc) or were not clustered with any fingerprints included in the database. Overall results obtained in the frame of the present work revealed that plants are inhabited by diverse and taxonomically rich Enterococcus spp. populations. This work was supported by the project CZ.1.07/2.3.00/20.0183.
ER  -

ŠVEC, Pavel, Lucie ZÁTOPKOVÁ, Hana BRYNDOVÁ a Ivo SEDLÁČEK. Characterization of Enterococcus species occuring on terrestrial vegetation. In \textit{International Union of Microbiological Societies Congresses (IUMS 2014)}. 2014.

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