LC-MS/MS determination of potential endocrine disruptors of
cortico signalling in rivers and wastewaters

J 2014

LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER; Marc J.F. SUTER et. al.

Basic information

Original name

LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters

Authors

AMMANN, Adrian A. (756 Switzerland, guarantor); Petra MACÍKOVÁ (203 Czech Republic, belonging to the institution); Ksenia J. GROH (756 Switzerland); Kristin SCHIRMER (756 Switzerland) and Marc J.F. SUTER (756 Switzerland)

Edition

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2014, 1618-2642

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10511 Environmental sciences

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 3.436

RIV identification code

RIV/00216224:14310/14:00079393

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00216-014-8206-9

UT WoS

000344860300024

EID Scopus

2-s2.0-84925510959

Keywords in English

Environmental endocrine disruptors; Corticosteroid signalling pathway; Pharmaceuticals; Metabolites; Surface water

Abstract

In the original language

A targeted analytical method was established to determine a large number of chemicals known to interfere with the gluco- and mineralocorticoid signalling pathway. The analytes comprise 30 glucocorticoids and 9 mineralocorticoids. Ten out of these corticosteroids were primary metabolites. Additionally, 14 nonsteroids were included. These analytes represent a broader range of possible adverse modes of action than previously reported. For the simultaneous determination of these structurally diverse compounds, a single-step multimode solid-phase extraction and pre-concentration was applied. Extracts were separated by a short linear HPLC gradient (20 min) on a core shell RP column (2.7 mu m particle size) and compounds identified and quantified by LC-MS/MS. The method provided excellent retention time reproducibility and detection limits in the low nanograms per litre range. Untreated hospital wastewater, wastewater treatment plant influent, treated effluent and river waters were analysed to demonstrate the applicability of the method. The results show that not all compounds were sufficiently eliminated by the wastewater treatment, resulting in the presence of several steroids (similar to 20 ng/L) and nonsteroids in the final effluent, some of them at high concentrations up to 200 ng/L. Most of the detected mono-hydroxylated steroidal transformation products were found at significantly higher concentrations than their parent compounds. We therefore recommend to include these potentially bioactive metabolites in environmental toxicity assessment.

Cite

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER and Marc J.F. SUTER. LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2014, vol. 406, No 29, p. 7653-7665. ISSN 1618-2642. Available from: https://dx.doi.org/10.1007/s00216-014-8206-9.

@article{1229204,
   author = {Ammann, Adrian A. and Macíková, Petra and Groh, Ksenia J. and Schirmer, Kristin and Suter, Marc J.F.},
   article_location = {HEIDELBERG},
   article_number = {29},
   doi = {http://dx.doi.org/10.1007/s00216-014-8206-9},
   keywords = {Environmental endocrine disruptors; Corticosteroid signalling pathway; Pharmaceuticals; Metabolites; Surface water},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters},
   url = {http://link.springer.com/article/10.1007%2Fs00216-014-8206-9},
   volume = {406},
   year = {2014}
}

TY  - JOUR
ID  - 1229204
AU  - Ammann, Adrian A. - Macíková, Petra - Groh, Ksenia J. - Schirmer, Kristin - Suter, Marc J.F.
PY  - 2014
TI  - LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters
JF  - Analytical and Bioanalytical chemistry
VL  - 406
IS  - 29
SP  - 7653-7665
EP  - 7653-7665
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Environmental endocrine disruptors
KW  - Corticosteroid signalling pathway
KW  - Pharmaceuticals
KW  - Metabolites
KW  - Surface water
UR  - http://link.springer.com/article/10.1007%2Fs00216-014-8206-9
L2  - http://link.springer.com/article/10.1007%2Fs00216-014-8206-9
N2  - A targeted analytical method was established to determine a large number of chemicals known to interfere with the gluco- and mineralocorticoid signalling pathway. The analytes comprise 30 glucocorticoids and 9 mineralocorticoids. Ten out of these corticosteroids were primary metabolites. Additionally, 14 nonsteroids were included. These analytes represent a broader range of possible adverse modes of action than previously reported. For the simultaneous determination of these structurally diverse compounds, a single-step multimode solid-phase extraction and pre-concentration was applied. Extracts were separated by a short linear HPLC gradient (20 min) on a core shell RP column (2.7 mu m particle size) and compounds identified and quantified by LC-MS/MS. The method provided excellent retention time reproducibility and detection limits in the low nanograms per litre range. Untreated hospital wastewater, wastewater treatment plant influent, treated effluent and river waters were analysed to demonstrate the applicability of the method. The results show that not all compounds were sufficiently eliminated by the wastewater treatment, resulting in the presence of several steroids (similar to 20 ng/L) and nonsteroids in the final effluent, some of them at high concentrations up to 200 ng/L. Most of the detected mono-hydroxylated steroidal transformation products were found at significantly higher concentrations than their parent compounds. We therefore recommend to include these potentially bioactive metabolites in environmental toxicity assessment.
ER  -

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER and Marc J.F. SUTER. LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2014, vol.~406, No~29, p.~7653-7665. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-014-8206-9.

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