Characterization of host-range mutations in genomes of
Staphylococcus Twortlikeviruses

J 2014

Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses

PANTŮČEK, Roman, Martin BENEŠÍK, Marian VARGA, Ivana MAŠLAŇOVÁ, Marek MOŠA et. al.

Základní údaje

Originální název

Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses

Autoři

PANTŮČEK, Roman (203 Česká republika, garant, domácí), Martin BENEŠÍK (203 Česká republika, domácí), Marian VARGA (203 Česká republika, domácí), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí), Marek MOŠA (203 Česká republika), Josef BOŠTÍK (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika, domácí)

Vydání

Russian Journal of Infection and Immunity, Sankt Peterburg, InformMed, 2014, 2220-7619

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Rusko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/14:00073314

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

bacteriophage therapy; Staphylococcus aureus; Twortlikevirus

Štítky

AKR, rivok

Změněno: 15. 4. 2015 16:01, Ing. Andrea Mikešková

Anotace

V originále

The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference.

Návaznosti

EE2.4.31.0155, projekt VaV

Název: Partnerství a sítě pro spolupráci v experimentální biologii

TA01010405, projekt VaV

Název: Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů (Akronym: TAČR/IMUNA-1)

Investor: Technologická agentura ČR, Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů

Citovat

PANTŮČEK, Roman, Martin BENEŠÍK, Marian VARGA, Ivana MAŠLAŇOVÁ, Marek MOŠA, Josef BOŠTÍK a Jiří DOŠKAŘ. Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses. Russian Journal of Infection and Immunity. Sankt Peterburg: InformMed, 2014, roč. 4, S1, s. 49-50. ISSN 2220-7619.

@article{1229242,
   author = {Pantůček, Roman and Benešík, Martin and Varga, Marian and Mašlaňová, Ivana and Moša, Marek and Boštík, Josef and Doškař, Jiří},
   article_location = {Sankt Peterburg},
   article_number = {S1},
   keywords = {bacteriophage therapy; Staphylococcus aureus; Twortlikevirus},
   language = {eng},
   issn = {2220-7619},
   journal = {Russian Journal of Infection and Immunity},
   title = {Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses},
   url = {http://iimmun.ru/index.php/iimm},
   volume = {4},
   year = {2014}
}

TY  - JOUR
ID  - 1229242
AU  - Pantůček, Roman - Benešík, Martin - Varga, Marian - Mašlaňová, Ivana - Moša, Marek - Boštík, Josef - Doškař, Jiří
PY  - 2014
TI  - Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses
JF  - Russian Journal of Infection and Immunity
VL  - 4
IS  - S1
SP  - 49-50
EP  - 49-50
PB  - InformMed
SN  - 22207619
KW  - bacteriophage therapy
KW  - Staphylococcus aureus
KW  - Twortlikevirus
UR  - http://iimmun.ru/index.php/iimm
N2  - The emergence and alarming increase of multiresistant strains of Staphylococcus aureus emphasizes the need for new and innovative antimicrobial strategies. Polyvalent bacteriophages from the family Myoviridae, sub-family Spounavirinae of genus Twort-like phages that infect S. aureus have potential for use in phage therapy. In this study we characterized genomic sequences of the polyvalent staphylococcal phage 812 and its spontaneous host-range mutants selected on non-susceptible S. aureus strains. The wild type phage was shown to lyse up to 65%, whereas host-range mutants up to 95% of S. aureus strains under study. Genomic DNAs of five phages were sequenced using 454 Genome Sequencer Junior (Roche). Bacteriophage DNA sequences were assembled by software GS De novo Assembler and GS Reference Mapper (Roche). Genome of phage K was chosen as the reference.
ER  -

PANTŮČEK, Roman, Martin BENEŠÍK, Marian VARGA, Ivana MAŠLAŇOVÁ, Marek MOŠA, Josef BOŠTÍK a Jiří DOŠKAŘ. Characterization of host-range mutations in genomes of Staphylococcus Twortlikeviruses. \textit{Russian Journal of Infection and Immunity}. Sankt Peterburg: InformMed, 2014, roč.~4, S1, s.~49-50. ISSN~2220-7619.

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