2014
Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany
HENNEBERG, A., K. BENDER, Luděk BLÁHA, S. GIEBNER, B. KUCH et. al.Základní údaje
Originální název
Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany
Autoři
HENNEBERG, A. (528 Nizozemské království, garant), K. BENDER (276 Německo), Luděk BLÁHA (203 Česká republika, domácí), S. GIEBNER (276 Německo), B. KUCH (276 Německo), H.R. KOHLER (528 Nizozemské království), D. MAIER (528 Nizozemské království), J. OEHLMANN (276 Německo), D. RICHTER (276 Německo), M. SCHEURER (276 Německo), U. SCHULTE-OEHLMANN (276 Německo), A. SIERATOWICZ (276 Německo), S. ZIEBART (276 Německo) a R. TRIEBSKORN (528 Nizozemské království)
Vydání
Plos one, San Francisco, Public Library of Science, 2014, 1932-6203
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10511 Environmental sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.234
Kód RIV
RIV/00216224:14310/14:00079907
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000336841400025
Klíčová slova anglicky
TREATMENT-PLANT EFFLUENTS; MUNICIPAL SEWAGE PLANT; UNITED-KINGDOM RIVERS; TROUT SALMO-TRUTTA; E-SCREEN ASSAY; POTAMOPYRGUS-ANTIPODARUM; ESTROGENIC ACTIVITY; WASTE-WATER; DISRUPTING CHEMICALS; BISPHENOL-A
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 31. 3. 2015 09:18, Ing. Filip Vaculovič
Anotace
V originále
Many studies about endocrine pollution in the aquatic environment reveal changes in the reproduction system of biota. We analysed endocrine activities in two rivers in Southern Germany using three approaches: (1) chemical analyses, (2) in vitro bioassays, and (3) in vivo investigations in fish and snails. Chemical analyses were based on gas chromatography coupled with mass spectrometry. For in vitro analyses of endocrine potentials in water, sediment, and waste water samples, we used the E-screen assay (human breast cancer cells MCF-7) and reporter gene assays (human cell line HeLa-9903 and MDA-kb2). In addition, we performed reproduction tests with the freshwater mudsnail Potamopyrgus antipodarum to analyse water and sediment samples. We exposed juvenile brown trout (Salmo trutta f. fario) to water downstream of a wastewater outfall (Schussen River) or to water from a reference site (Argen River) to investigate the vitellogenin production. Furthermore, two feral fish species, chub (Leuciscus cephalus) and spirlin (Alburnoides bipunctatus), were caught in both rivers to determine their gonadal maturity and the gonadosomatic index. Chemical analyses provided only little information about endocrine active substances, whereas the in vitro assays revealed endocrine potentials in most of the samples. In addition to endocrine potentials, we also observed toxic potentials (E-screen/reproduction test) in waste water samples, which could interfere with and camouflage endocrine effects. The results of our in vivo tests were mostly in line with the results of the in vitro assays and revealed a consistent reproduction-disrupting (reproduction tests) and an occasional endocrine action (vitellogenin levels) in both investigated rivers, with more pronounced effects for the Schussen river (e. g. a lower gonadosomatic index). We were able to show that biological in vitro assays for endocrine potentials in natural stream water reasonably reflect reproduction and endocrine disruption observed in snails and field-exposed fish, respectively.