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@article{1232612, author = {Henneberg, A. and Bender, K. and Bláha, Luděk and Giebner, S. and Kuch, B. and Kohler, H.R. and Maier, D. and Oehlmann, J. and Richter, D. and Scheurer, M. and SchulteandOehlmann, U. and Sieratowicz, A. and Ziebart, S. and Triebskorn, R.}, article_location = {San Francisco}, article_number = {6}, doi = {http://dx.doi.org/10.1371/journal.pone.0098307}, keywords = {TREATMENT-PLANT EFFLUENTS; MUNICIPAL SEWAGE PLANT; UNITED-KINGDOM RIVERS; TROUT SALMO-TRUTTA; E-SCREEN ASSAY; POTAMOPYRGUS-ANTIPODARUM; ESTROGENIC ACTIVITY; WASTE-WATER; DISRUPTING CHEMICALS; BISPHENOL-A}, language = {eng}, issn = {1932-6203}, journal = {Plos one}, title = {Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany}, url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098307}, volume = {9}, year = {2014} }
TY - JOUR ID - 1232612 AU - Henneberg, A. - Bender, K. - Bláha, Luděk - Giebner, S. - Kuch, B. - Kohler, H.R. - Maier, D. - Oehlmann, J. - Richter, D. - Scheurer, M. - Schulte-Oehlmann, U. - Sieratowicz, A. - Ziebart, S. - Triebskorn, R. PY - 2014 TI - Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany JF - Plos one VL - 9 IS - 6 SP - 1-16 EP - 1-16 PB - Public Library of Science SN - 19326203 KW - TREATMENT-PLANT EFFLUENTS KW - MUNICIPAL SEWAGE PLANT KW - UNITED-KINGDOM RIVERS KW - TROUT SALMO-TRUTTA KW - E-SCREEN ASSAY KW - POTAMOPYRGUS-ANTIPODARUM KW - ESTROGENIC ACTIVITY KW - WASTE-WATER KW - DISRUPTING CHEMICALS KW - BISPHENOL-A UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098307 N2 - Many studies about endocrine pollution in the aquatic environment reveal changes in the reproduction system of biota. We analysed endocrine activities in two rivers in Southern Germany using three approaches: (1) chemical analyses, (2) in vitro bioassays, and (3) in vivo investigations in fish and snails. Chemical analyses were based on gas chromatography coupled with mass spectrometry. For in vitro analyses of endocrine potentials in water, sediment, and waste water samples, we used the E-screen assay (human breast cancer cells MCF-7) and reporter gene assays (human cell line HeLa-9903 and MDA-kb2). In addition, we performed reproduction tests with the freshwater mudsnail Potamopyrgus antipodarum to analyse water and sediment samples. We exposed juvenile brown trout (Salmo trutta f. fario) to water downstream of a wastewater outfall (Schussen River) or to water from a reference site (Argen River) to investigate the vitellogenin production. Furthermore, two feral fish species, chub (Leuciscus cephalus) and spirlin (Alburnoides bipunctatus), were caught in both rivers to determine their gonadal maturity and the gonadosomatic index. Chemical analyses provided only little information about endocrine active substances, whereas the in vitro assays revealed endocrine potentials in most of the samples. In addition to endocrine potentials, we also observed toxic potentials (E-screen/reproduction test) in waste water samples, which could interfere with and camouflage endocrine effects. The results of our in vivo tests were mostly in line with the results of the in vitro assays and revealed a consistent reproduction-disrupting (reproduction tests) and an occasional endocrine action (vitellogenin levels) in both investigated rivers, with more pronounced effects for the Schussen river (e. g. a lower gonadosomatic index). We were able to show that biological in vitro assays for endocrine potentials in natural stream water reasonably reflect reproduction and endocrine disruption observed in snails and field-exposed fish, respectively. ER -
HENNEBERG, A., K. BENDER, Luděk BLÁHA, S. GIEBNER, B. KUCH, H.R. KOHLER, D. MAIER, J. OEHLMANN, D. RICHTER, M. SCHEURER, U. SCHULTE-OEHLMANN, A. SIERATOWICZ, S. ZIEBART a R. TRIEBSKORN. Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany. \textit{Plos one}. San Francisco: Public Library of Science, 2014, roč.~9, č.~6, s.~1-16. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0098307.
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