2014
Inflammatory profiling of Schwann cells in contact with growing axons distal to nerve injury
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁZákladní údaje
Originální název
Inflammatory profiling of Schwann cells in contact with growing axons distal to nerve injury
Název česky
Zánětlivá profilace Schwanových buněk v kontaktu s rostoucími axony distálně od poškození periferního nervu
Autoři
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ
Vydání
International Symposium on Peripheral Nerve Regeneration, Torino, 2014
Další údaje
Jazyk
angličtina
Typ výsledku
Vyžádané přednášky
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Itálie
Utajení
není předmětem státního či obchodního tajemství
Klíčová slova česky
peripheral neuropatic pain model, Wallerian degeneration, activated Schwann cells, cytokines, macrophages
Klíčová slova anglicky
model periferní neuropatické bolesti, Wallerova degenerace, aktivované Schwannovy buňky, cytokiny, makrofágy
Změněno: 31. 3. 2015 13:13, MUDr. Ilona Klusáková, Ph.D.
Anotace
V originále
Wallerian degeneration is a cascade of stereotype cellular and molecular events distal to injury of nerve fibers considered to be a sort of innate immune reaction or neuroinflammation. The cellular events comprise inflammatory activation of Schwann cells and invasion of various types of immune cells including macrophages that produce cytokines and chemokines. However, little is known on beneficial effect of proinflammatory cytokines upregulated by activated Schwann cells. Therefore, the goal of present study was to investigate expression of proinflammatory cytokines (IL-1b, TNFa, IL-6) and their receptors in activated Schwann cells distal to crush and compression injury of rat sciatic nerves.The sciatic nerve sections from naïve or sham-operated rats displayed very week immunostaining for investigated cytokines and their receptors. Immunofluorescence of significantly higher intensity was found in sections distal to nerve lesion as well as in sections of contralateral sciatic nerve. Immunostaining revealed that proinflammatory cytokines and their receptors are dominantly expressed by activated Schwann cells which many of them were in close contact with growing axons. Surprisingly, activated ED-1+ macrophages in distal sciatic nerve segments displayed significantly lower level of proinflammatory cytokines and their receptors.
Návaznosti
| CZ.1.05/1.1.00/02.0068, interní kód MU |
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| CZ.1.07/2.4.00/17.0042, interní kód MU |
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