Localized movement and morphology of UBF1-positive nucleolar
regions are changed by gamma-irradiation in G2 phase of the
cell cycle

J 2015

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ et. al.

Základní údaje

Originální název

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

Autoři

SOROKIN, Dmitry (643 Rusko, garant, domácí), Lenka STIXOVÁ (203 Česká republika), Petra SEHNALOVÁ (203 Česká republika), Soňa LEGARTOVÁ (703 Slovensko), Jana SUCHÁNKOVÁ (203 Česká republika), Pavel ŠIMARA (203 Česká republika, domácí), Stanislav KOZUBEK (203 Česká republika), Pavel MATULA (203 Česká republika, domácí), Magdalena SKALNÍKOVÁ (203 Česká republika), Ivan RAŠKA (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Nucleus, 2015, 1949-1034

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.446

Kód RIV

RIV/00216224:14330/15:00080738

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1080/19491034.2015.1075111

UT WoS

000360666000008

Klíčová slova anglicky

DNA damage; live cells; nucleolus; uncleoli tracking; UBF1

Štítky

CBIA, cbia-web

Změněno: 28. 4. 2016 13:56, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that gradiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Pavel ŠIMARA, Stanislav KOZUBEK, Pavel MATULA, Magdalena SKALNÍKOVÁ, Ivan RAŠKA a Eva BÁRTOVÁ. Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle. Nucleus. 2015, roč. 6, č. 4, s. 301-313. ISSN 1949-1034. Dostupné z: https://dx.doi.org/10.1080/19491034.2015.1075111.

@article{1232675,
   author = {Sorokin, Dmitry and Stixová, Lenka and Sehnalová, Petra and Legartová, Soňa and Suchánková, Jana and Šimara, Pavel and Kozubek, Stanislav and Matula, Pavel and Skalníková, Magdalena and Raška, Ivan and Bártová, Eva},
   article_number = {4},
   doi = {http://dx.doi.org/10.1080/19491034.2015.1075111},
   keywords = {DNA damage; live cells; nucleolus; uncleoli tracking; UBF1},
   language = {eng},
   issn = {1949-1034},
   journal = {Nucleus},
   title = {Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1232675
AU  - Sorokin, Dmitry - Stixová, Lenka - Sehnalová, Petra - Legartová, Soňa - Suchánková, Jana - Šimara, Pavel - Kozubek, Stanislav - Matula, Pavel - Skalníková, Magdalena - Raška, Ivan - Bártová, Eva
PY  - 2015
TI  - Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle
JF  - Nucleus
VL  - 6
IS  - 4
SP  - 301-313
EP  - 301-313
SN  - 19491034
KW  - DNA damage
KW  - live cells
KW  - nucleolus
KW  - uncleoli tracking
KW  - UBF1
N2  - The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that gradiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.
ER  -

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Pavel ŠIMARA, Stanislav KOZUBEK, Pavel MATULA, Magdalena SKALNÍKOVÁ, Ivan RAŠKA a Eva BÁRTOVÁ. Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle. \textit{Nucleus}. 2015, roč.~6, č.~4, s.~301-313. ISSN~1949-1034. Dostupné z: https://dx.doi.org/10.1080/19491034.2015.1075111.

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