A novel panel of proteins correlating with lymph node
metastasis of low-grade breast cancer as identified by combined
proteomics and transcriptomics approach

a 2014

A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Ivana IHNATOVÁ et. al.

Basic information

Original name

A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach

Authors

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Ivana IHNATOVÁ; Iva STRUHÁROVÁ; Eva BUDINSKÁ; Spiros D. GARBIS; Bořivoj VOJTĚŠEK and Rudolf NENUTIL

Edition

2014

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

10600 1.6 Biological sciences

Country of publisher

Spain

Confidentiality degree

is not subject to a state or trade secret

Organization unit

Faculty of Science

Keywords in English

breast cancer; proteomics; biomarkers

Abstract

In the original language

Introduction and objectives Current prognostic factors are not sufficient for precise risk discrimination in all groups of breast cancer patients. This is evident in the case of low grade breast tumors the small percentage of which, in disagreement with favorable prognosis, forms early lymph node metastasis. New molecular targets and biomarkers of this phenomenon are thus of high clinical need. Material and Methods We focused on identification of low grade specific, metastasis correlating proteins in the set of 48 small (T1) grade 1 luminal A primary breast tumors. Another set of 48 high grade tumors of various subtypes was analyzed to investigate the specificity of identified gene products. 4405 proteins were identified (FDR < 5%) within iTRAQ-2DLC-MS/MS proteomics analysis. Expression of 95 selected genes was analyzed at transcript level. Levels of top 5 proteins were analyzed also using immunohistochemistry. Connection of gene expression and patient survival was investigated in completely independent set of 214 breast tumors. Results and Discussion The panel of identified proteins correlating with lymph node positivity low grade breast tumors at protein and transcript level, supported by similar trends in immunohistochemistry and patient survival data, involved activators of NF-κB pathway, secreted protease and cytoskeletal proteins. Analysis of biomarker selectivity divided the potential biomarkers into two selectivity groups: (i) Gene products up-regulated specifically in lymph node positive low grade tumors, (ii) those that had, in addition, higher levels in another groups of aggressive tumors (high grade and triple negative breast cancer). Conclusions The group of identified functionally relevant biomarkers in now in the process of further validation and deeper characterization of their role in breast cancer metastasis.

Links

GA14-19250S, research and development project

Name: Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk

Investor: Czech Science Foundation

Cite

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Ivana IHNATOVÁ; Iva STRUHÁROVÁ; Eva BUDINSKÁ; Spiros D. GARBIS; Bořivoj VOJTĚŠEK and Rudolf NENUTIL. A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach. 2014.

@proceedings{1232826,
   author = {Bouchal, Pavel and Dvořáková, Monika and Roumeliotis, Theodoros and Bortlíček, Zbyněk and Ihnatová, Ivana and Struhárová, Iva and Budinská, Eva and Garbis, Spiros D. and Vojtěšek, Bořivoj and Nenutil, Rudolf},
   keywords = {breast cancer; proteomics; biomarkers},
   language = {eng},
   title = {A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach},
   year = {2014}
}

TY  - CONF
ID  - 1232826
AU  - Bouchal, Pavel - Dvořáková, Monika - Roumeliotis, Theodoros - Bortlíček, Zbyněk - Ihnatová, Ivana - Struhárová, Iva - Budinská, Eva - Garbis, Spiros D. - Vojtěšek, Bořivoj - Nenutil, Rudolf
PY  - 2014
TI  - A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach
KW  - breast cancer
KW  - proteomics
KW  - biomarkers
N2  - Introduction and objectives Current prognostic factors are not sufficient for precise risk discrimination in all groups of breast cancer patients. This is evident in the case of low grade breast tumors the small percentage of which, in disagreement with favorable prognosis, forms early lymph node metastasis. New molecular targets and biomarkers of this phenomenon are thus of high clinical need. Material and Methods We focused on identification of low grade specific, metastasis correlating proteins in the set of 48 small (T1) grade 1 luminal A primary breast tumors. Another set of 48 high grade tumors of various subtypes was analyzed to investigate the specificity of identified gene products. 4405 proteins were identified (FDR < 5%) within iTRAQ-2DLC-MS/MS proteomics analysis. Expression of 95 selected genes was analyzed at transcript level. Levels of top 5 proteins were analyzed also using immunohistochemistry. Connection of gene expression and patient survival was investigated in completely independent set of 214 breast tumors. Results and Discussion The panel of identified proteins correlating with lymph node positivity low grade breast tumors at protein and transcript level, supported by similar trends in immunohistochemistry and patient survival data, involved activators of NF-κB pathway, secreted protease and cytoskeletal proteins. Analysis of biomarker selectivity divided the potential biomarkers into two selectivity groups: (i) Gene products up-regulated specifically in lymph node positive low grade tumors, (ii) those that had, in addition, higher levels in another groups of aggressive tumors (high grade and triple negative breast cancer). Conclusions The group of identified functionally relevant biomarkers in now in the process of further validation and deeper characterization of their role in breast cancer metastasis.
ER  -

BOUCHAL, Pavel; Monika DVOŘÁKOVÁ; Theodoros ROUMELIOTIS; Zbyněk BORTLÍČEK; Ivana IHNATOVÁ; Iva STRUHÁROVÁ; Eva BUDINSKÁ; Spiros D. GARBIS; Bořivoj VOJTĚŠEK and Rudolf NENUTIL. \textit{A novel panel of proteins correlating with lymph node metastasis of low-grade breast cancer as identified by combined proteomics and transcriptomics approach}. 2014.

Přehled o publikaci