Cell differentiation of lignified tissues is controlled by
cytokinins via negative regulation of master regulators NST1
and NST3

a 2015

Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ et. al.

Základní údaje

Originální název

Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3

Autoři

DIDI, Vojtěch (203 Česká republika, domácí), Jana VAŠÍČKOVÁ (203 Česká republika), Radek JUPA (203 Česká republika, domácí), Mariana BENITEZ KEINRAD (484 Mexiko, domácí), Tereza DOBISOVÁ (203 Česká republika, domácí), Radim ČEGAN (203 Česká republika), Roman HOBZA (203 Česká republika, domácí), Shawn MANSFIELD (124 Kanada), Vít GLOSER (203 Česká republika, domácí) a Jan HEJÁTKO (203 Česká republika, domácí)

Vydání

Keystone Symphosia on Molecular and Cellular Biology - Plant Receptor Kinases: From Molecules to Environment, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14740/15:00080755

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Secondary cell wall development; Cytokinins; Cell differentiation

Změněno: 17. 4. 2015 16:46, Mgr. Vojtěch Didi, Ph.D.

Anotace

V originále

Control over the equilibrium between cell division and cell differentiation represents the core developmental regulation across all kingdoms. Plant hormones cytokinins (CKs) and CK-regulated sensor histidine kinases were shown to regulate the process of cell differentiation in both the shoot and root. However, the molecular mechanism of CK-controlled cell differentiation is far from being understood. We found that impaired CK signalling or biosynthesis leads to formation of vascular bundles with changed protoxylem/metaxylem ratio and dramatically reduced water conductance. Increased lignification in both tracheary elements (TEs) and xylem fibers suggested disturbed cell differentiation. The genome-wide next-gen transcriptional profiling revealed upregulation throughout the hierarchical cascade of transcriptional regulations including the NAC TFs NST1 and SND1/NST3, the master transcriptional switches of secondary cell wall (SCW) formation acting at the top of the cascade. That leads to precocious activation of downstream structural components mediating the biosynthesis of xylan, lignin and cellulose, the main compounds of the SCW. Using promoter:GUS lines, complementation of CK biosynthetic mutants by exogenous CK application and manipulation of endogenous CK via CYTOKININ OXIDASE/DEHYDROGENASE overexpression we have proven that CKs control proper cell differentiation rate via negative control over NST1 and NST3. Altogether our data provide mechanistic framework describing the role of CKs as principal regulators of cell differentiation program in plants.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-25280S, projekt VaV

Název: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Grantová agentura ČR, Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Citovat

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ, Radim ČEGAN, Roman HOBZA, Shawn MANSFIELD, Vít GLOSER a Jan HEJÁTKO. Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3. In Keystone Symphosia on Molecular and Cellular Biology - Plant Receptor Kinases: From Molecules to Environment. 2015.

@proceedings{1298040,
   author = {Didi, Vojtěch and Vašíčková, Jana and Jupa, Radek and Benitez Keinrad, Mariana and Dobisová, Tereza and Čegan, Radim and Hobza, Roman and Mansfield, Shawn and Gloser, Vít and Hejátko, Jan},
   booktitle = {Keystone Symphosia on Molecular and Cellular Biology - Plant Receptor Kinases: From Molecules to Environment},
   keywords = {Secondary cell wall development; Cytokinins; Cell differentiation},
   language = {eng},
   title = {Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3},
   year = {2015}
}

TY  - CONF
ID  - 1298040
AU  - Didi, Vojtěch - Vašíčková, Jana - Jupa, Radek - Benitez Keinrad, Mariana - Dobisová, Tereza - Čegan, Radim - Hobza, Roman - Mansfield, Shawn - Gloser, Vít - Hejátko, Jan
PY  - 2015
TI  - Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3
KW  - Secondary cell wall development
KW  - Cytokinins
KW  - Cell differentiation
N2  - Control over the equilibrium between cell division and cell differentiation represents the core developmental regulation across all kingdoms. Plant hormones cytokinins (CKs) and CK-regulated sensor histidine kinases were shown to regulate the process of cell differentiation in both the shoot and root. However, the molecular mechanism of CK-controlled cell differentiation is far from being understood. We found that impaired CK signalling or biosynthesis leads to formation of vascular bundles with changed protoxylem/metaxylem ratio and dramatically reduced water conductance. Increased lignification in both tracheary elements (TEs) and xylem fibers suggested disturbed cell differentiation. The genome-wide next-gen transcriptional profiling revealed upregulation throughout the hierarchical cascade of transcriptional regulations including the NAC TFs NST1 and SND1/NST3, the master transcriptional switches of secondary cell wall (SCW) formation acting at the top of the cascade. That leads to precocious activation of downstream structural components mediating the biosynthesis of xylan, lignin and cellulose, the main compounds of the SCW. Using promoter:GUS lines, complementation of CK biosynthetic mutants by exogenous CK application and manipulation of endogenous CK via CYTOKININ OXIDASE/DEHYDROGENASE overexpression we have proven that CKs control proper cell differentiation rate via negative control over NST1 and NST3. Altogether our data provide mechanistic framework describing the role of CKs as principal regulators of cell differentiation program in plants.
ER  -

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ, Radim ČEGAN, Roman HOBZA, Shawn MANSFIELD, Vít GLOSER a Jan HEJÁTKO. Cell differentiation of lignified tissues is controlled by cytokinins via negative regulation of master regulators NST1 and NST3. In \textit{Keystone Symphosia on Molecular and Cellular Biology - Plant Receptor Kinases: From Molecules to Environment}. 2015.

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