2015
Genetic Architecture of Natural Variation of Telomere Length in Arabidopsis thaliana
FULCHER, Nick, Astrid TEUBENBACHER, Envel KERDAFFREC, Ashley FARLOW, Magnus NORDBORG et. al.Základní údaje
Originální název
Genetic Architecture of Natural Variation of Telomere Length in Arabidopsis thaliana
Autoři
FULCHER, Nick (40 Rakousko), Astrid TEUBENBACHER (40 Rakousko), Envel KERDAFFREC (40 Rakousko), Ashley FARLOW (40 Rakousko), Magnus NORDBORG (40 Rakousko) a Karel ŘÍHA (203 Česká republika, garant, domácí)
Vydání
Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2015, 0016-6731
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 4.644
Kód RIV
RIV/00216224:14740/15:00082746
Organizační jednotka
Středoevropský technologický institut
UT WoS
000349459400026
Klíčová slova anglicky
telomere; QTL; centromere-mediated genome elimination; haploid; Arabidopsis
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 23. 3. 2016 10:50, Mgr. Eva Špillingová
Anotace
V originále
Telomeres represent the repetitive sequences that cap chromosome ends and are essential for their protection. Telomere length is known to be highly heritable and is derived from a homeostatic balance between telomeric lengthening and shortening activities. Specific loci that form the genetic framework underlying telomere length homeostasis, however, are not well understood. To investigate the extent of natural variation of telomere length in Arabidopsis thaliana, we examined 229 worldwide accessions by terminal restriction fragment analysis. The results showed a wide range of telomere lengths that are specific to individual accessions. To identify loci that are responsible for this variation, we adopted a quantitative trait loci (QTL) mapping approach with multiple recombinant inbred line (RIL) populations. A doubled haploid RIL population was first produced using centromere-mediated genome elimination between accessions with long (Pro-0) and intermediate (Col-0) telomere lengths. Composite interval mapping analysis of this population along with two established RIL populations (Ler-2/Cvi-0 and Est-1/Col-0) revealed a number of shared and unique QTL. QTL detected in the Ler-2/Cvi-0 population were examined using near isogenic lines that confirmed causative regions on chromosomes 1 and 2. In conclusion, this work describes the extent of natural variation of telomere length in A. thaliana, identifies a network of QTL that influence telomere length homeostasis, examines telomere length dynamics in plants with hybrid backgrounds, and shows the effects of two identified regions on telomere length regulation.