Blood - cerebrospinal fluid barrier in rat after peripheral
nerve injury

a 2015

Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury

JOUKAL, Marek, Peter SOLÁR, Adéla KUKLOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ et. al.

Základní údaje

Originální název

Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury

Název česky

Hematolikvorová bariéra laboratorního potkana po poškození periferního nervu

Autoři

JOUKAL, Marek, Peter SOLÁR, Adéla KUKLOVÁ, Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Vydání

CEITEC PHD RETREAT, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

978-80-210-7825-3

Klíčová slova česky

Hematolikvorová bariéra, poškození periferního nervu, FluoroEmerald, imunohistochemie

Klíčová slova anglicky

blood - cerebrospinal fluid berrier, peripheral nerve injury, FluoroEmerald, imunohistochemistry

Změněno: 25. 4. 2015 09:41, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

The unilateral chronical constriction injury (CCI) used as a neuropathic pain model causes the elevation of proinflammatory cytokines levels not only in the dorsal root ganglia (DRG) related to damaged nerve, but also in remote DRG and central nervous system structures. We purpose that one of the possible pathways for spread of neuroinflammation could be over the blood-cerebrospinal fluid barrier present in choroid plexus (CP). We used the intravenous injection of fluorescent conjugated dextrane (FluoroEmerald - FE) to clarify our hypothesis. Twelve Wistar male rats were divided to the 3 days (n=5) and 21 days (n=5) of CCI groups, two rats were naive. The FE was intravenously applied at time of CCI survival and rats were sacrificed after 18 hours of FE circulation, perfused transcardially by Zamboni´s fixative. Distribution of FE and simultaneous immunohistochemical detection for resident (ED2) and activated (ED1) macrophages, antigen presenting cells (APC; MHC-II) and microglia (OX-42) was studied in CP using coronal cryostat sections through the brain. FluoroEmerald particles were presented in cuboidal epithelial cells of CP. The activated macrophages, microglia and APC positive for FE were found in CP stroma. Epiplexal Kolmer cells immunostained for ED2 and MHC-II were loaded by FE. In addition, FE particles were also found in ventricular ependymal cells (EC). Our results suggest that composite effect of CCI and FE causes immune reaction in CP followed by diffusion of FE particles into EC.

Citovat

JOUKAL, Marek, Peter SOLÁR, Adéla KUKLOVÁ, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury. In CEITEC PHD RETREAT. 2015. ISBN 978-80-210-7825-3.

@proceedings{1298409,
   author = {Joukal, Marek and Solár, Peter and Kuklová, Adéla and Klusáková, Ilona and Dubový, Petr},
   booktitle = {CEITEC PHD RETREAT},
   keywords = {blood - cerebrospinal fluid berrier, peripheral nerve injury, FluoroEmerald, imunohistochemistry},
   language = {eng},
   isbn = {978-80-210-7825-3},
   title = {Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury},
   year = {2015}
}

TY  - CONF
ID  - 1298409
AU  - Joukal, Marek - Solár, Peter - Kuklová, Adéla - Klusáková, Ilona - Dubový, Petr
PY  - 2015
TI  - Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury
SN  - 9788021078253
KW  - blood - cerebrospinal fluid berrier, peripheral nerve injury, FluoroEmerald, imunohistochemistry
N2  - The unilateral chronical constriction injury (CCI) used as a neuropathic pain model causes the elevation of proinflammatory cytokines levels not only in the dorsal root ganglia (DRG) related to damaged nerve, but also in remote DRG and central nervous system structures. We purpose that one of the possible pathways for spread of neuroinflammation could be over the blood-cerebrospinal fluid barrier present in choroid plexus (CP). We used the intravenous injection of fluorescent conjugated dextrane (FluoroEmerald - FE) to clarify our hypothesis. Twelve Wistar male rats were divided to the 3 days (n=5) and 21 days (n=5) of CCI groups, two rats were naive. The FE was intravenously applied at time of CCI survival and rats were sacrificed after 18 hours of FE circulation, perfused transcardially by Zamboni´s fixative. Distribution of FE and simultaneous immunohistochemical detection for resident (ED2) and activated (ED1) macrophages, antigen presenting cells (APC; MHC-II) and microglia (OX-42) was studied in CP using coronal cryostat sections through the brain. FluoroEmerald particles were presented in cuboidal epithelial cells of CP. The activated macrophages, microglia and APC positive for FE were found in CP stroma. Epiplexal Kolmer cells immunostained for ED2 and MHC-II were loaded by FE. In addition, FE particles were also found in ventricular ependymal cells (EC). Our results suggest that composite effect of CCI and FE causes immune reaction in CP followed by diffusion of FE particles into EC.
ER  -

JOUKAL, Marek, Peter SOLÁR, Adéla KUKLOVÁ, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Blood - cerebrospinal fluid barrier in rat after peripheral nerve injury. In \textit{CEITEC PHD RETREAT}. 2015. ISBN~978-80-210-7825-3.

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