2015
Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T
BEDNAŘÍK, Petr; Amir MOHEET; Dinesh K. DEELCHAND; Uzay E. EMIR; Lynn E. EBERLY et. al.Základní údaje
Originální název
Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T
Autoři
BEDNAŘÍK, Petr (203 Česká republika, garant, domácí); Amir MOHEET (840 Spojené státy); Dinesh K. DEELCHAND (840 Spojené státy); Uzay E. EMIR (840 Spojené státy); Lynn E. EBERLY (840 Spojené státy); Martin BAREŠ (203 Česká republika, domácí); Elizabeth R. SEAQUIST (840 Spojené státy) a Guelin OZ (840 Spojené státy)
Vydání
NMR in Biomedicine, Hoboken (USA), Wiley-Blackwell, 2015, 0952-3480
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10610 Biophysics
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.983
Kód RIV
RIV/00216224:14740/15:00082759
Organizační jednotka
Středoevropský technologický institut
UT WoS
000354416300010
EID Scopus
2-s2.0-84929276614
Klíčová slova anglicky
3T; MRS; coefficient of variation; human hippocampus; metabolites; quantification precision; reproducibility; segmentation
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 23. 3. 2016 11:00, Mgr. Eva Špillingová
Anotace
V originále
Hippocampal dysfunction is known to be associated with several neurological and neuropsychiatric disorders such as Alzheimer's disease, epilepsy, schizophrenia and depression; therefore, there has been significant clinical interest in studying hippocampal neurochemistry. However, the hippocampus is a challenging region to study using H-1 MRS, hence the use of MRS for clinical research in this region has been limited. Our goal was therefore to investigate the feasibility of obtaining high-quality hippocampal spectra that allow reliable quantification of a neurochemical profile and to establish inter-session reproducibility of hippocampal MRS, including reproducibility of voxel placement, spectral quality and neurochemical concentrations. Ten healthy volunteers were scanned in two consecutive sessions using a standard clinical 3T MR scanner. Neurochemical profiles were obtained with a short-echo (T-E=28ms) semi-LASER localization sequence from a relatively small (similar to 4mL) voxel that covered about 62% of the hippocampal volume as calculated from segmentation of T-1-weighted images. Voxel composition was highly reproducible between sessions, with test-retest coefficients of variation (CVs) of 3.5% and 7.5% for gray and white matter volume fraction, respectively. Excellent signal-to-noise ratio (similar to 54 based on the N-acetylaspartate (NAA) methyl peak in non-apodized spectra) and linewidths (similar to 9Hz for water) were achieved reproducibly in all subjects. The spectral quality allowed quantification of NAA, total choline, total creatine, myo-inositol and glutamate with high scan-rescan reproducibility (CV6%) and quantification precision (Cramer-Rao lower bound, CRLB<9%). Four other metabolites, including glutathione and glucose, were quantified with scan-rescan CV below 20%. Therefore, the highly optimized, short-echo semi-LASER sequence together with FASTMAP shimming substantially improved the reproducibility and number of quantifiable metabolites relative to prior reports. In addition, the between-session variation in metabolite concentrations, as well as CRLB, was lower than the between-subject variation of the concentrations for most metabolites, indicating that the method has the sensitivity to detect inter-individual differences in the healthy brain. Copyright (c) 2015 John Wiley & Sons, Ltd.
Návaznosti
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