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@article{1298434,
author = {Bednařík, Petr and Moheet, Amir and Deelchand, Dinesh K. and Emir, Uzay E. and Eberly, Lynn E. and Bareš, Martin and Seaquist, Elizabeth R. and Oz, Guelin},
article_location = {Hoboken (USA)},
article_number = {6},
doi = {http://dx.doi.org/10.1002/nbm.3309},
keywords = {3T; MRS; coefficient of variation; human hippocampus; metabolites; quantification precision; reproducibility; segmentation},
language = {eng},
issn = {0952-3480},
journal = {NMR in Biomedicine},
title = {Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T},
url = {http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf},
volume = {28},
year = {2015}
}
TY - JOUR
ID - 1298434
AU - Bednařík, Petr - Moheet, Amir - Deelchand, Dinesh K. - Emir, Uzay E. - Eberly, Lynn E. - Bareš, Martin - Seaquist, Elizabeth R. - Oz, Guelin
PY - 2015
TI - Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T
JF - NMR in Biomedicine
VL - 28
IS - 6
SP - 685-693
EP - 685-693
PB - Wiley-Blackwell
SN - 09523480
KW - 3T
KW - MRS
KW - coefficient of variation
KW - human hippocampus
KW - metabolites
KW - quantification precision
KW - reproducibility
KW - segmentation
UR - http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf
L2 - http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf
N2 - Hippocampal dysfunction is known to be associated with several neurological and neuropsychiatric disorders such as Alzheimer's disease, epilepsy, schizophrenia and depression; therefore, there has been significant clinical interest in studying hippocampal neurochemistry. However, the hippocampus is a challenging region to study using H-1 MRS, hence the use of MRS for clinical research in this region has been limited. Our goal was therefore to investigate the feasibility of obtaining high-quality hippocampal spectra that allow reliable quantification of a neurochemical profile and to establish inter-session reproducibility of hippocampal MRS, including reproducibility of voxel placement, spectral quality and neurochemical concentrations. Ten healthy volunteers were scanned in two consecutive sessions using a standard clinical 3T MR scanner. Neurochemical profiles were obtained with a short-echo (T-E=28ms) semi-LASER localization sequence from a relatively small (similar to 4mL) voxel that covered about 62% of the hippocampal volume as calculated from segmentation of T-1-weighted images. Voxel composition was highly reproducible between sessions, with test-retest coefficients of variation (CVs) of 3.5% and 7.5% for gray and white matter volume fraction, respectively. Excellent signal-to-noise ratio (similar to 54 based on the N-acetylaspartate (NAA) methyl peak in non-apodized spectra) and linewidths (similar to 9Hz for water) were achieved reproducibly in all subjects. The spectral quality allowed quantification of NAA, total choline, total creatine, myo-inositol and glutamate with high scan-rescan reproducibility (CV6%) and quantification precision (Cramer-Rao lower bound, CRLB<9%). Four other metabolites, including glutathione and glucose, were quantified with scan-rescan CV below 20%. Therefore, the highly optimized, short-echo semi-LASER sequence together with FASTMAP shimming substantially improved the reproducibility and number of quantifiable metabolites relative to prior reports. In addition, the between-session variation in metabolite concentrations, as well as CRLB, was lower than the between-subject variation of the concentrations for most metabolites, indicating that the method has the sensitivity to detect inter-individual differences in the healthy brain. Copyright (c) 2015 John Wiley & Sons, Ltd.
ER -
BEDNAŘÍK, Petr, Amir MOHEET, Dinesh K. DEELCHAND, Uzay E. EMIR, Lynn E. EBERLY, Martin BAREŠ, Elizabeth R. SEAQUIST a Guelin OZ. Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T. \textit{NMR in Biomedicine}. Hoboken (USA): Wiley-Blackwell, 2015, roč.~28, č.~6, s.~685-693. ISSN~0952-3480. Dostupné z: https://dx.doi.org/10.1002/nbm.3309.
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