Další formáty:
BibTeX
LaTeX
RIS
@article{1298434, author = {Bednařík, Petr and Moheet, Amir and Deelchand, Dinesh K. and Emir, Uzay E. and Eberly, Lynn E. and Bareš, Martin and Seaquist, Elizabeth R. and Oz, Guelin}, article_location = {Hoboken (USA)}, article_number = {6}, doi = {http://dx.doi.org/10.1002/nbm.3309}, keywords = {3T; MRS; coefficient of variation; human hippocampus; metabolites; quantification precision; reproducibility; segmentation}, language = {eng}, issn = {0952-3480}, journal = {NMR in Biomedicine}, title = {Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T}, url = {http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf}, volume = {28}, year = {2015} }
TY - JOUR ID - 1298434 AU - Bednařík, Petr - Moheet, Amir - Deelchand, Dinesh K. - Emir, Uzay E. - Eberly, Lynn E. - Bareš, Martin - Seaquist, Elizabeth R. - Oz, Guelin PY - 2015 TI - Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T JF - NMR in Biomedicine VL - 28 IS - 6 SP - 685-693 EP - 685-693 PB - Wiley-Blackwell SN - 09523480 KW - 3T KW - MRS KW - coefficient of variation KW - human hippocampus KW - metabolites KW - quantification precision KW - reproducibility KW - segmentation UR - http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf L2 - http://onlinelibrary.wiley.com/doi/10.1002/nbm.3309/epdf N2 - Hippocampal dysfunction is known to be associated with several neurological and neuropsychiatric disorders such as Alzheimer's disease, epilepsy, schizophrenia and depression; therefore, there has been significant clinical interest in studying hippocampal neurochemistry. However, the hippocampus is a challenging region to study using H-1 MRS, hence the use of MRS for clinical research in this region has been limited. Our goal was therefore to investigate the feasibility of obtaining high-quality hippocampal spectra that allow reliable quantification of a neurochemical profile and to establish inter-session reproducibility of hippocampal MRS, including reproducibility of voxel placement, spectral quality and neurochemical concentrations. Ten healthy volunteers were scanned in two consecutive sessions using a standard clinical 3T MR scanner. Neurochemical profiles were obtained with a short-echo (T-E=28ms) semi-LASER localization sequence from a relatively small (similar to 4mL) voxel that covered about 62% of the hippocampal volume as calculated from segmentation of T-1-weighted images. Voxel composition was highly reproducible between sessions, with test-retest coefficients of variation (CVs) of 3.5% and 7.5% for gray and white matter volume fraction, respectively. Excellent signal-to-noise ratio (similar to 54 based on the N-acetylaspartate (NAA) methyl peak in non-apodized spectra) and linewidths (similar to 9Hz for water) were achieved reproducibly in all subjects. The spectral quality allowed quantification of NAA, total choline, total creatine, myo-inositol and glutamate with high scan-rescan reproducibility (CV6%) and quantification precision (Cramer-Rao lower bound, CRLB<9%). Four other metabolites, including glutathione and glucose, were quantified with scan-rescan CV below 20%. Therefore, the highly optimized, short-echo semi-LASER sequence together with FASTMAP shimming substantially improved the reproducibility and number of quantifiable metabolites relative to prior reports. In addition, the between-session variation in metabolite concentrations, as well as CRLB, was lower than the between-subject variation of the concentrations for most metabolites, indicating that the method has the sensitivity to detect inter-individual differences in the healthy brain. Copyright (c) 2015 John Wiley & Sons, Ltd. ER -
BEDNAŘÍK, Petr, Amir MOHEET, Dinesh K. DEELCHAND, Uzay E. EMIR, Lynn E. EBERLY, Martin BAREŠ, Elizabeth R. SEAQUIST a Guelin OZ. Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3T. \textit{NMR in Biomedicine}. Hoboken (USA): Wiley-Blackwell, 2015, roč.~28, č.~6, s.~685-693. ISSN~0952-3480. Dostupné z: https://dx.doi.org/10.1002/nbm.3309.
|