Acinetobacter seifertii sp nov., a member of the Acinetobacter
calcoaceticus-Acinetobacter baumannii complex isolated from
human clinical specimens

J 2015

Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens

NEMEC, Alexandr, Lenka KŘÍŽOVÁ, Martina MAIXNEROVÁ, Ondrej ŠEDO, Sylvain BRISSE et. al.

Základní údaje

Originální název

Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens

Autoři

NEMEC, Alexandr (203 Česká republika), Lenka KŘÍŽOVÁ (203 Česká republika), Martina MAIXNEROVÁ (203 Česká republika), Ondrej ŠEDO (203 Česká republika, garant, domácí), Sylvain BRISSE (250 Francie) a Paul G. HIGGINS (276 Německo)

Vydání

International Journal of Systematic and Evolutionary Microbiology, Berks (England), Society for General Microbiology, 2015, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.439

Kód RIV

RIV/00216224:14740/15:00080811

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1099/ijs.0.000043

UT WoS

000353670100030

Klíčová slova anglicky

GENUS ACINETOBACTER; IDENTIFICATION

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2016 15:37, Mgr. Eva Špillingová

Anotace

V originále

This study aimed to define the taxonomic status of a phenetically distinct group of 16 strains that corresponds to Acinetobacter genonnic species 'close to 13TU', a provisional genomic species of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex recognized by Gerner-Smidt and Tjernberg in 1993. These strains have been isolated in different countries since the early 1990s and were mostly recovered from human clinical specimens. They were compared with 45 reference strains representing the known taxa of the ACB complex using taxonomic methods relevant to the genus Acinetobacter. Based on sequence analysis of the concatenated partial sequences (2976 bp) of seven housekeeping genes, the 16 strains formed a tight and well-supported cluster (intracluster sequence identity of >= 98.4 %) that was clearly separated from the other members of the ACB complex (<= 94.7%). The species status of the group was supported by average nucleotide identity values of <= 91.7 % between the whole genome sequence of representative strain NIPH 973(T) (NCBI accession no. AP0000000000) and those of the other species. In addition, whole-cell matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) MS analyses indicated the distinctness of the group at the protein level. Metabolic and physiological tests revealed several typical features of the group, although they did not allow its reliable differentiation from the other members of the ACB complex. We conclude that the 16 strains represent a distinct novel species, for which we propose the name Acinetobacter seifertii sp. nov. The type strain is NIPH 973(T) (=CIP 110471(T)=CCUG 34785(T)=CCM 8535(T)).

Návaznosti

GA13-26693S, projekt VaV

Název: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Grantová agentura ČR, Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech

Citovat

NEMEC, Alexandr, Lenka KŘÍŽOVÁ, Martina MAIXNEROVÁ, Ondrej ŠEDO, Sylvain BRISSE a Paul G. HIGGINS. Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. International Journal of Systematic and Evolutionary Microbiology. Berks (England): Society for General Microbiology, 2015, roč. 65, č. 3, s. 934-942. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.000043.

@article{1299778,
   author = {Nemec, Alexandr and Křížová, Lenka and Maixnerová, Martina and Šedo, Ondrej and Brisse, Sylvain and Higgins, Paul G.},
   article_location = {Berks (England)},
   article_number = {3},
   doi = {http://dx.doi.org/10.1099/ijs.0.000043},
   keywords = {GENUS ACINETOBACTER; IDENTIFICATION},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens},
   url = {http://ijs.sgmjournals.org/content/65/Pt_3/934.full.pdf+html},
   volume = {65},
   year = {2015}
}

TY  - JOUR
ID  - 1299778
AU  - Nemec, Alexandr - Křížová, Lenka - Maixnerová, Martina - Šedo, Ondrej - Brisse, Sylvain - Higgins, Paul G.
PY  - 2015
TI  - Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 65
IS  - 3
SP  - 934-942
EP  - 934-942
PB  - Society for General Microbiology
SN  - 14665026
KW  - GENUS ACINETOBACTER
KW  - IDENTIFICATION
UR  - http://ijs.sgmjournals.org/content/65/Pt_3/934.full.pdf+html
L2  - http://ijs.sgmjournals.org/content/65/Pt_3/934.full.pdf+html
N2  - This study aimed to define the taxonomic status of a phenetically distinct group of 16 strains that corresponds to Acinetobacter genonnic species 'close to 13TU', a provisional genomic species of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex recognized by Gerner-Smidt and Tjernberg in 1993. These strains have been isolated in different countries since the early 1990s and were mostly recovered from human clinical specimens. They were compared with 45 reference strains representing the known taxa of the ACB complex using taxonomic methods relevant to the genus Acinetobacter. Based on sequence analysis of the concatenated partial sequences (2976 bp) of seven housekeeping genes, the 16 strains formed a tight and well-supported cluster (intracluster sequence identity of >= 98.4 %) that was clearly separated from the other members of the ACB complex (<= 94.7%). The species status of the group was supported by average nucleotide identity values of <= 91.7 % between the whole genome sequence of representative strain NIPH 973(T) (NCBI accession no. AP0000000000) and those of the other species. In addition, whole-cell matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) MS analyses indicated the distinctness of the group at the protein level. Metabolic and physiological tests revealed several typical features of the group, although they did not allow its reliable differentiation from the other members of the ACB complex. We conclude that the 16 strains represent a distinct novel species, for which we propose the name Acinetobacter seifertii sp. nov. The type strain is NIPH 973(T) (=CIP 110471(T)=CCUG 34785(T)=CCM 8535(T)).
ER  -

NEMEC, Alexandr, Lenka KŘÍŽOVÁ, Martina MAIXNEROVÁ, Ondrej ŠEDO, Sylvain BRISSE a Paul G. HIGGINS. Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. \textit{International Journal of Systematic and Evolutionary Microbiology}. Berks (England): Society for General Microbiology, 2015, roč.~65, č.~3, s.~934-942. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.000043.

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