LATTOVÁ, Erika, Adéla BRABCOVÁ, Veronika BÁRTOVÁ, David POTĚŠIL, Jan BÁRTA a Zbyněk ZDRÁHAL. N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. Washington D.C.: American Chemical Society, 2015, roč. 63, č. 12, s. 3243-3250. ISSN 0021-8561. Dostupné z: https://dx.doi.org/10.1021/acs.jafc.5b00426. |
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@article{1299826, author = {Lattová, Erika and Brabcová, Adéla and Bártová, Veronika and Potěšil, David and Bárta, Jan and Zdráhal, Zbyněk}, article_location = {Washington D.C.}, article_number = {12}, doi = {http://dx.doi.org/10.1021/acs.jafc.5b00426}, keywords = {glycans; mass speetrometry; patatin; plants; potato; Solanum}, language = {eng}, issn = {0021-8561}, journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, title = {N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus}, url = {http://pubs.acs.org/doi/pdf/10.1021/acs.jafc.5b00426}, volume = {63}, year = {2015} }
TY - JOUR ID - 1299826 AU - Lattová, Erika - Brabcová, Adéla - Bártová, Veronika - Potěšil, David - Bárta, Jan - Zdráhal, Zbyněk PY - 2015 TI - N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus JF - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY VL - 63 IS - 12 SP - 3243-3250 EP - 3243-3250 PB - American Chemical Society SN - 00218561 KW - glycans KW - mass speetrometry KW - patatin KW - plants KW - potato KW - Solanum UR - http://pubs.acs.org/doi/pdf/10.1021/acs.jafc.5b00426 L2 - http://pubs.acs.org/doi/pdf/10.1021/acs.jafc.5b00426 N2 - It is hypothesized that oligosaccharides are another potential source of immunological cross-reaction between different plant allergens. Patatin is the most abundant glycoprotein in potato and has been described to have an oligosaccharide of composition Man3(Xyl)GlcNAc2(Fuc). In this work, N-glycosylation profiles of patatin proteins isolated from tubers of different potato species were investigated and compared. Oligosaccharides were released by enzymatic digestion with PNAGase A and analyzed primarily by matrix-assisted laser desorption ionization mass spectrometry. For glycan labeling, a modified version of on-target derivatization with phenylhydrazine was applied. This study found the presence of glycan structures not described previously in patatins of potato tubers, and their glycan profiles significantly differed. This knowledge about the glycosylation of potato patatins may be helpful for correct choice of potato species to decrease the presence of specific glycan epitopes causing food allergy as well as for utilization of potatoes for the manufacture of therapeutic proteins. ER -
LATTOVÁ, Erika, Adéla BRABCOVÁ, Veronika BÁRTOVÁ, David POTĚŠIL, Jan BÁRTA a Zbyněk ZDRÁHAL. N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus. \textit{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}. Washington D.C.: American Chemical Society, 2015, roč.~63, č.~12, s.~3243-3250. ISSN~0021-8561. Dostupné z: https://dx.doi.org/10.1021/acs.jafc.5b00426.
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