Quartz crystal microbalance biosensor for rapid detection of
aerosolized microorganisms

D 2015

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

FARKA, Zdeněk; David KOVÁŘ and Petr SKLÁDAL

Basic information

Original name

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution); David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

USA, Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, p. 1-9, 9 pp. 2015

Publisher

SPIE

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14740/15:00082954

Organization unit

Central European Institute of Technology

ISBN

978-1-62841-571-1

ISSN

DOI

https://doi.org/10.1117/12.2084606

UT WoS

000357744700006

EID Scopus

2-s2.0-84948844220

Keywords in English

Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli

Abstract

In the original language

Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Cite

FARKA, Zdeněk; David KOVÁŘ and Petr SKLÁDAL. Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms. Online. In Augustus Way Fountain. Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI. USA: SPIE, 2015, p. 1-9. ISBN 978-1-62841-571-1. Available from: https://doi.org/10.1117/12.2084606.

@inproceedings{1299970,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   address = {USA},
   booktitle = {Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI},
   doi = {https://doi.org/10.1117/12.2084606},
   editor = {Augustus Way Fountain},
   keywords = {Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {USA},
   isbn = {978-1-62841-571-1},
   pages = {1-9},
   publisher = {SPIE},
   title = {Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms},
   url = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2300142},
   year = {2015}
}

TY  - CONF
ID  - 1299970
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2015
TI  - Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
PB  - SPIE
CY  - USA
SN  - 9781628415711
KW  - Biosensor
KW  - Cyclone sampling
KW  - Quartz crystal microbalance
KW  - Bioaerosol
KW  - Escherichia coli
UR  - http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2300142
N2  - Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.
ER  -

FARKA, Zdeněk; David KOVÁŘ and Petr SKLÁDAL. Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms. Online. In Augustus Way Fountain. \textit{Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI}. USA: SPIE, 2015, p.~1-9. ISBN~978-1-62841-571-1. Available from: https://doi.org/10.1117/12.2084606.

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