| AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ, Václav NĚMEC, Veronika LIŠKOVÁ, Nagendra Prasad KURUMBANG, Koen BEERENS, Radka CHALOUPKOVÁ, Kamil PARUCH, Martina HOF a Jiří DAMBORSKÝ. Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. Journal of the American Chemical Society. 2015, roč. 137, č. 15, s. 4988-4992. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.5b01681. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1300082,
author = {Amaro, M. and Brezovský, Jan and Kováčová, Silvia and Sykora, J. and Bednář, David and Němec, Václav and Lišková, Veronika and Kurumbang, Nagendra Prasad and Beerens, Koen and Chaloupková, Radka and Paruch, Kamil and Hof, Martina and Damborský, Jiří},
article_number = {15},
doi = {http://dx.doi.org/10.1021/jacs.5b01681},
keywords = {dehalogenases;fluorescence spectroscopy;molecular dynamics simulations},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.},
url = {http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf},
volume = {137},
year = {2015}
}
TY - JOUR
ID - 1300082
AU - Amaro, M. - Brezovský, Jan - Kováčová, Silvia - Sykora, J. - Bednář, David - Němec, Václav - Lišková, Veronika - Kurumbang, Nagendra Prasad - Beerens, Koen - Chaloupková, Radka - Paruch, Kamil - Hof, Martina - Damborský, Jiří
PY - 2015
TI - Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
JF - Journal of the American Chemical Society
VL - 137
IS - 15
SP - 4988-4992
EP - 4988-4992
SN - 00027863
KW - dehalogenases;fluorescence spectroscopy;molecular dynamics simulations
UR - http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf
L2 - http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf
N2 - Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
ER -
AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ, Václav NĚMEC, Veronika LIŠKOVÁ, Nagendra Prasad KURUMBANG, Koen BEERENS, Radka CHALOUPKOVÁ, Kamil PARUCH, Martina HOF a Jiří DAMBORSKÝ. Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. \textit{Journal of the American Chemical Society}. 2015, roč.~137, č.~15, s.~4988-4992. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.5b01681.
|
