Calprotectin and lactoferrin in the cerebrospinal fluid;
biomarkers utilisable for differential diagnostics of bacterial
and aseptic meningitis?

J 2015

Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?

DASTYCH, Milan; Jana GOTTWALDOVÁ a Zdeňka ČERMÁKOVÁ

Základní údaje

Originální název

Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?

Autoři

DASTYCH, Milan; Jana GOTTWALDOVÁ a Zdeňka ČERMÁKOVÁ

Vydání

Clinical Chemistry and Laboratory medicine, BERLIN, WALTER DE GRUYTER & CO, 2015, 1434-6621

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.017

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00082982

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

bacterial meningitis; calprotectin; cerebrospinal fluid; lactoferrin

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 5. 2015 14:47, Soňa Böhmová

Anotace

V originále

Background: The aim of our work was to assess the diagnostic contribution of calprotectin and lactoferrin determinations in the cerebrospinal fluid when distinguishing between bacterial and aseptic meningitides. Methods: In 23 patients with bacterial meningitis (BM) and in 50 patients with aseptic meningitis (AM), we determined the concentrations of calprotectin, lactoferrin and the conventional biomarkers like glucose, total protein, lactate and polynuclear count in the cerebrospinal fluid (CSF). The discriminative power of the various parameters studied was determined by means of receiver operating characteristic (ROC) curves: the area under the curve (AUC), sensitivity, specificity, the positive likelihood ratio (+LR), and the negative likelihood ratio (-LR). Results: The diagnostic efficiency of calprotectin, lactoferrin, lactate, and polynuclear count when distinguishing between bacterial and aseptic meningitides, expressed by ROC curve parameters, was as follows: AUC (0.736, 0.946, 0.932, 0.932), sensitivity (86.2, 96.6, 90.0, 89.7), specificity (58.5, 92.4, 87.0, 90.6), +LR (2.08, 12.8, 6.9, 9.50), -LR (0.24, 0.04, 0.11, 0.11), respectively. The optimal cut point for calprotectin and lactoferrin was 191 ng/mL and 17.8 ng/mL, respectively. Conclusions: Our findings show, that the determination of lactoferrin in the CSF was diagnostically the most efficient marker in distinguishing between bacterial and viral meningitides. Calprotectin was far less efficient diagnostic marker. The polynuclear count and lactate concentration showed a very good diagnostic efficiency as well. The determination of protein and glucose was diagnostically less beneficial.

Citovat

DASTYCH, Milan; Jana GOTTWALDOVÁ a Zdeňka ČERMÁKOVÁ. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis? Clinical Chemistry and Laboratory medicine. BERLIN: WALTER DE GRUYTER & CO, 2015, roč. 53, č. 4, s. 599-603. ISSN 1434-6621. Dostupné z: https://doi.org/10.1515/cclm-2014-0775.

@article{1300093,
   author = {Dastych, Milan and Gottwaldová, Jana and Čermáková, Zdeňka},
   article_location = {BERLIN},
   article_number = {4},
   doi = {https://doi.org/10.1515/cclm-2014-0775},
   keywords = {bacterial meningitis; calprotectin; cerebrospinal fluid; lactoferrin},
   language = {eng},
   issn = {1434-6621},
   journal = {Clinical Chemistry and Laboratory medicine},
   title = {Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?},
   volume = {53},
   year = {2015}
}

TY  - JOUR
ID  - 1300093
AU  - Dastych, Milan - Gottwaldová, Jana - Čermáková, Zdeňka
PY  - 2015
TI  - Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
JF  - Clinical Chemistry and Laboratory medicine
VL  - 53
IS  - 4
SP  - 599-603
EP  - 599-603
PB  - WALTER DE GRUYTER & CO
SN  - 14346621
KW  - bacterial meningitis
KW  - calprotectin
KW  - cerebrospinal fluid
KW  - lactoferrin
N2  - Background: The aim of our work was to assess the diagnostic contribution of calprotectin and lactoferrin determinations in the cerebrospinal fluid when distinguishing between bacterial and aseptic meningitides. Methods: In 23 patients with bacterial meningitis (BM) and in 50 patients with aseptic meningitis (AM), we determined the concentrations of calprotectin, lactoferrin and the conventional biomarkers like glucose, total protein, lactate and polynuclear count in the cerebrospinal fluid (CSF). The discriminative power of the various parameters studied was determined by means of receiver operating characteristic (ROC) curves: the area under the curve (AUC), sensitivity, specificity, the positive likelihood ratio (+LR), and the negative likelihood ratio (-LR). Results: The diagnostic efficiency of calprotectin, lactoferrin, lactate, and polynuclear count when distinguishing between bacterial and aseptic meningitides, expressed by ROC curve parameters, was as follows: AUC (0.736, 0.946, 0.932, 0.932), sensitivity (86.2, 96.6, 90.0, 89.7), specificity (58.5, 92.4, 87.0, 90.6), +LR (2.08, 12.8, 6.9, 9.50), -LR (0.24, 0.04, 0.11, 0.11), respectively. The optimal cut point for calprotectin and lactoferrin was 191 ng/mL and 17.8 ng/mL, respectively. Conclusions: Our findings show, that the determination of lactoferrin in the CSF was diagnostically the most efficient marker in distinguishing between bacterial and viral meningitides. Calprotectin was far less efficient diagnostic marker. The polynuclear count and lactate concentration showed a very good diagnostic efficiency as well. The determination of protein and glucose was diagnostically less beneficial.
ER  -

DASTYCH, Milan; Jana GOTTWALDOVÁ a Zdeňka ČERMÁKOVÁ. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis? \textit{Clinical Chemistry and Laboratory medicine}. BERLIN: WALTER DE GRUYTER \&{} CO, 2015, roč.~53, č.~4, s.~599-603. ISSN~1434-6621. Dostupné z: https://doi.org/10.1515/cclm-2014-0775.

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