Calprotectin and lactoferrin in the cerebrospinal fluid;
biomarkers utilisable for differential diagnostics of bacterial
and aseptic meningitis?

J 2015

Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?

DASTYCH, Milan; Jana GOTTWALDOVÁ and Zdeňka ČERMÁKOVÁ

Basic information

Original name

Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?

Authors

DASTYCH, Milan (203 Czech Republic, guarantor, belonging to the institution); Jana GOTTWALDOVÁ (203 Czech Republic, belonging to the institution) and Zdeňka ČERMÁKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Clinical Chemistry and Laboratory medicine, BERLIN, WALTER DE GRUYTER & CO, 2015, 1434-6621

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.017

RIV identification code

RIV/00216224:14110/15:00082982

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1515/cclm-2014-0775

UT WoS

000350674500021

EID Scopus

2-s2.0-84925813797

Keywords in English

bacterial meningitis; calprotectin; cerebrospinal fluid; lactoferrin

Abstract

In the original language

Background: The aim of our work was to assess the diagnostic contribution of calprotectin and lactoferrin determinations in the cerebrospinal fluid when distinguishing between bacterial and aseptic meningitides. Methods: In 23 patients with bacterial meningitis (BM) and in 50 patients with aseptic meningitis (AM), we determined the concentrations of calprotectin, lactoferrin and the conventional biomarkers like glucose, total protein, lactate and polynuclear count in the cerebrospinal fluid (CSF). The discriminative power of the various parameters studied was determined by means of receiver operating characteristic (ROC) curves: the area under the curve (AUC), sensitivity, specificity, the positive likelihood ratio (+LR), and the negative likelihood ratio (-LR). Results: The diagnostic efficiency of calprotectin, lactoferrin, lactate, and polynuclear count when distinguishing between bacterial and aseptic meningitides, expressed by ROC curve parameters, was as follows: AUC (0.736, 0.946, 0.932, 0.932), sensitivity (86.2, 96.6, 90.0, 89.7), specificity (58.5, 92.4, 87.0, 90.6), +LR (2.08, 12.8, 6.9, 9.50), -LR (0.24, 0.04, 0.11, 0.11), respectively. The optimal cut point for calprotectin and lactoferrin was 191 ng/mL and 17.8 ng/mL, respectively. Conclusions: Our findings show, that the determination of lactoferrin in the CSF was diagnostically the most efficient marker in distinguishing between bacterial and viral meningitides. Calprotectin was far less efficient diagnostic marker. The polynuclear count and lactate concentration showed a very good diagnostic efficiency as well. The determination of protein and glucose was diagnostically less beneficial.

Cite

DASTYCH, Milan; Jana GOTTWALDOVÁ and Zdeňka ČERMÁKOVÁ. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis? Clinical Chemistry and Laboratory medicine. BERLIN: WALTER DE GRUYTER & CO, 2015, vol. 53, No 4, p. 599-603. ISSN 1434-6621. Available from: https://doi.org/10.1515/cclm-2014-0775.

@article{1300093,
   author = {Dastych, Milan and Gottwaldová, Jana and Čermáková, Zdeňka},
   article_location = {BERLIN},
   article_number = {4},
   doi = {https://doi.org/10.1515/cclm-2014-0775},
   keywords = {bacterial meningitis; calprotectin; cerebrospinal fluid; lactoferrin},
   language = {eng},
   issn = {1434-6621},
   journal = {Clinical Chemistry and Laboratory medicine},
   title = {Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?},
   volume = {53},
   year = {2015}
}

TY  - JOUR
ID  - 1300093
AU  - Dastych, Milan - Gottwaldová, Jana - Čermáková, Zdeňka
PY  - 2015
TI  - Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
JF  - Clinical Chemistry and Laboratory medicine
VL  - 53
IS  - 4
SP  - 599-603
EP  - 599-603
PB  - WALTER DE GRUYTER & CO
SN  - 14346621
KW  - bacterial meningitis
KW  - calprotectin
KW  - cerebrospinal fluid
KW  - lactoferrin
N2  - Background: The aim of our work was to assess the diagnostic contribution of calprotectin and lactoferrin determinations in the cerebrospinal fluid when distinguishing between bacterial and aseptic meningitides. Methods: In 23 patients with bacterial meningitis (BM) and in 50 patients with aseptic meningitis (AM), we determined the concentrations of calprotectin, lactoferrin and the conventional biomarkers like glucose, total protein, lactate and polynuclear count in the cerebrospinal fluid (CSF). The discriminative power of the various parameters studied was determined by means of receiver operating characteristic (ROC) curves: the area under the curve (AUC), sensitivity, specificity, the positive likelihood ratio (+LR), and the negative likelihood ratio (-LR). Results: The diagnostic efficiency of calprotectin, lactoferrin, lactate, and polynuclear count when distinguishing between bacterial and aseptic meningitides, expressed by ROC curve parameters, was as follows: AUC (0.736, 0.946, 0.932, 0.932), sensitivity (86.2, 96.6, 90.0, 89.7), specificity (58.5, 92.4, 87.0, 90.6), +LR (2.08, 12.8, 6.9, 9.50), -LR (0.24, 0.04, 0.11, 0.11), respectively. The optimal cut point for calprotectin and lactoferrin was 191 ng/mL and 17.8 ng/mL, respectively. Conclusions: Our findings show, that the determination of lactoferrin in the CSF was diagnostically the most efficient marker in distinguishing between bacterial and viral meningitides. Calprotectin was far less efficient diagnostic marker. The polynuclear count and lactate concentration showed a very good diagnostic efficiency as well. The determination of protein and glucose was diagnostically less beneficial.
ER  -

DASTYCH, Milan; Jana GOTTWALDOVÁ and Zdeňka ČERMÁKOVÁ. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis? \textit{Clinical Chemistry and Laboratory medicine}. BERLIN: WALTER DE GRUYTER \&{} CO, 2015, vol.~53, No~4, p.~599-603. ISSN~1434-6621. Available from: https://doi.org/10.1515/cclm-2014-0775.

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