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@article{1300332,
author = {Verner, Jan and Trbušek, Martin and Chovancová, Jana and Jašková, Zuzana and Moulis, Mojmír and Folber, František and Halouzka, Roman and Mayer, Jiří and Pospíšilová, Šárka and Doubek, Michael},
article_location = {Abingdon},
article_number = {11},
doi = {http://dx.doi.org/10.3109/10428194.2015.1034701},
keywords = {chronic lymphocytic leukemia; mantle cell lymphoma; NSG mouse; xenograft model},
language = {eng},
issn = {1042-8194},
journal = {Leukemia & Lymphoma},
title = {NOD/SCID IL2R gamma-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines},
volume = {56},
year = {2015}
}
TY - JOUR
ID - 1300332
AU - Verner, Jan - Trbušek, Martin - Chovancová, Jana - Jašková, Zuzana - Moulis, Mojmír - Folber, František - Halouzka, Roman - Mayer, Jiří - Pospíšilová, Šárka - Doubek, Michael
PY - 2015
TI - NOD/SCID IL2R gamma-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines
JF - Leukemia & Lymphoma
VL - 56
IS - 11
SP - 3198-3206
EP - 3198-3206
PB - Taylor & Francis LTD
SN - 10428194
KW - chronic lymphocytic leukemia
KW - mantle cell lymphoma
KW - NSG mouse
KW - xenograft model
N2 - Xenograft models represent a promising tool to study the pathogenesis of hematological malignancies. To establish a reliable and appropriate in vivo model of aggressive human B-cell leukemia and lymphoma we xenotransplanted four p53-mutated cell lines and one ATM-mutated cell line into immunodeficient NOD/SCID IL2R gamma-null mice. The cell lines MEC-1, SU-DHL-4, JEKO-1, REC-1, and GRANTA-519 were transplanted intraperitoneally or subcutaneously and the engraftment was investigated using immunohistochemistry and flow cytometry. We found significant differences in engraftment efficiency. MEC-1, JEKO-1 and GRANTA-519 cell lines engrafted most efficiently, while SU-DHL-4 cells did not engraft at all. MEC-1 and GRANTA-519 massively infiltrated organs and the whole intraperitoneal cavity showing very aggressive growth. In addition, GRANTA-519 cells massively migrated to the bone marrow regardless of the transplantation route. The MEC-1 and GRANTA-519 cells can be especially recommended for in vivo study of p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma, respectively.
ER -
VERNER, Jan, Martin TRBUŠEK, Jana CHOVANCOVÁ, Zuzana JAŠKOVÁ, Mojmír MOULIS, František FOLBER, Roman HALOUZKA, Jiří MAYER, Šárka POSPÍŠILOVÁ a Michael DOUBEK. NOD/SCID IL2R gamma-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines. \textit{Leukemia \&{} Lymphoma}. Abingdon: Taylor \&{} Francis LTD, 2015, roč.~56, č.~11, s.~3198-3206. ISSN~1042-8194. Dostupné z: https://dx.doi.org/10.3109/10428194.2015.1034701.
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