| KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Petr VAŇHARA, Aleš HAMPL a Josef HAVEL. Use of flower-like gold nanoparticles in time-of-flight mass spectrometry. Rapid Communication in Mass Spectrometry. Hoboken: Wiley-Blackwell, 2015, roč. 29, č. 17, s. 1585-1595. ISSN 0951-4198. Dostupné z: https://dx.doi.org/10.1002/rcm.7265. |
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@article{1301571,
author = {Kolářová, Lenka and Kučera, Lukáš and Vaňhara, Petr and Hampl, Aleš and Havel, Josef},
article_location = {Hoboken},
article_number = {17},
doi = {http://dx.doi.org/10.1002/rcm.7265},
keywords = {Flower-like gold nanoparticles; MALDI; SALDI; TOF mass spectrometry; biomolecules},
language = {eng},
issn = {0951-4198},
journal = {Rapid Communication in Mass Spectrometry},
title = {Use of flower-like gold nanoparticles in time-of-flight mass spectrometry},
volume = {29},
year = {2015}
}
TY - JOUR
ID - 1301571
AU - Kolářová, Lenka - Kučera, Lukáš - Vaňhara, Petr - Hampl, Aleš - Havel, Josef
PY - 2015
TI - Use of flower-like gold nanoparticles in time-of-flight mass spectrometry
JF - Rapid Communication in Mass Spectrometry
VL - 29
IS - 17
SP - 1585-1595
EP - 1585-1595
PB - Wiley-Blackwell
SN - 09514198
KW - Flower-like gold nanoparticles
KW - MALDI
KW - SALDI
KW - TOF mass spectrometry
KW - biomolecules
N2 - Many kinds of nanoparticles (NPs) have been used for mass spectrometry (MS) so far. Here we report the first use of flower-like gold nanoparticles (AuNPs) as a mediator enhancing ionization in MS of peptides and proteins. Flower-like AuNPs were characterized using transmission and scanning electron microscopy, UV-VIS spectrophotometry, and laser desorption/ionization (LDI) MS and compared with polyhedral AuNPs. Mass spectra were obtained in positive ion mode using time-of-flight (TOF) analyzer coupled with either matrix assisted laser desorption/ionization (MALDI) or surface assisted laser desorption/ionization (SALDI) methods. The intensities of peptide peaks (m/z 500–3500) were up to 7.5× and up to 7× higher using flower-like AuNPs and flower-like AuNPs-enriched cyano-4-hydroxycinnamic acid (CHCA) matrix respectively, than the classical CHCA matrix. The signals of higher mass peptide/protein peaks (m/z 3600–17000) were up to 2× higher with using flower-like AuNPs enriched CHCA matrix than conventional CHCA matrix. The signal of profile peaks generated by intact cell MALDI-TOFMS of fibroblast suspension (m/z 4000–20000) was 2× higher with using flower-like AuNPs combined with sinapinic acid (SA) compared to SA matrix alone. The use of flower-like AuNPs as internal calibration standard for the calibration of MS spectra of peptides was performed. Flower-like AuNPs and flower-like AuNPs combined with CHCA or SA as combined matrices for MS measurement of peptides and proteins were used. Comparison of conventional MALDI method and our method with flower-like AuNPs was carried out. In addition, gold clusters generated from flower-like AuNPs by SALDI provide suitable internal calibration standard for MS analysis of peptides.
ER -
KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Petr VAŇHARA, Aleš HAMPL a Josef HAVEL. Use of flower-like gold nanoparticles in time-of-flight mass spectrometry. \textit{Rapid Communication in Mass Spectrometry}. Hoboken: Wiley-Blackwell, 2015, roč.~29, č.~17, s.~1585-1595. ISSN~0951-4198. Dostupné z: https://dx.doi.org/10.1002/rcm.7265.
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