Unique C. elegans telomeric overhang structures reveal the
evolutionarily conserved properties of telomeric DNA

J 2015

Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA

ŠKOLÁKOVÁ, Petra, Silvie TRANTÍRKOVÁ, Klára BEDNÁŘOVÁ, Radovan FIALA, Michaela VORLÍČKOVÁ et. al.

Základní údaje

Originální název

Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA

Autoři

ŠKOLÁKOVÁ, Petra (203 Česká republika), Silvie TRANTÍRKOVÁ (203 Česká republika, domácí), Klára BEDNÁŘOVÁ (203 Česká republika, domácí), Radovan FIALA (203 Česká republika, domácí), Michaela VORLÍČKOVÁ (203 Česká republika, garant, domácí) a Lukáš TRANTÍREK (203 Česká republika, domácí)

Vydání

Nucleic Acids Research, Oxford, UK, Oxford Press, 2015, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.202

Kód RIV

RIV/00216224:14740/15:00080881

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/nar/gkv296

UT WoS

000355928800035

Klíčová slova anglicky

NUCLEASE HYPERSENSITIVE ELEMENT; G-QUADRUPLEX STRUCTURES; I-MOTIF; CAENORHABDITIS-ELEGANS; HYDROGEN-BONDS; CELL-LINES; BASE-PAIRS; CATIONIC PORPHYRINS; CIRCULAR-DICHROISM; HUMAN-CHROMOSOMES

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 7. 2015 09:08, Martina Prášilová

Anotace

V originále

There are two basic mechanisms that are associated with the maintenance of the telomere length, which endows cancer cells with unlimited proliferative potential. One mechanism, referred to as alternative lengthening of telomeres (ALT), accounts for approximately 10-15% of all human cancers. Tumours engaged in the ALT pathway are characterised by the presence of the single stranded 5'-C-rich telomeric overhang (C-overhang). This recently identified hallmark of ALT cancers distinguishes them from healthy tissues and renders the C-overhang as a clear target for anticancer therapy. We analysed structures of the 5'-C-rich and 3'-G-rich telomeric overhangs from human and Caenorhabditis elegans, the recently established multicellular in vivo model of ALT tumours. We show that the telomeric DNA from C. elegans and humans forms fundamentally different secondary structures. The unique structural characteristics of C. elegans telomeric DNA that are distinct not only from those of humans but also from those of other multicellular eukaryotes allowed us to identify evolutionarily conserved properties of telomeric DNA. Differences in structural organisation of the telomeric DNA between the C. elegans and human impose limitations on the use of the C. elegans as an ALT tumour model.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-28310S, projekt VaV

Název: Evolučně konzervované strukturní vlastnosti centromerické a telomerické DNA

Investor: Grantová agentura ČR, Evolucne konzervované strukturní vlastnosti centromerické a telomerické DNA

Citovat

ŠKOLÁKOVÁ, Petra, Silvie TRANTÍRKOVÁ, Klára BEDNÁŘOVÁ, Radovan FIALA, Michaela VORLÍČKOVÁ a Lukáš TRANTÍREK. Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA. Nucleic Acids Research. Oxford, UK: Oxford Press, 2015, roč. 43, č. 9, s. 4733-4745. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv296.

@article{1305254,
   author = {Školáková, Petra and Trantírková, Silvie and Bednářová, Klára and Fiala, Radovan and Vorlíčková, Michaela and Trantírek, Lukáš},
   article_location = {Oxford, UK},
   article_number = {9},
   doi = {http://dx.doi.org/10.1093/nar/gkv296},
   keywords = {NUCLEASE HYPERSENSITIVE ELEMENT; G-QUADRUPLEX STRUCTURES; I-MOTIF; CAENORHABDITIS-ELEGANS; HYDROGEN-BONDS; CELL-LINES; BASE-PAIRS; CATIONIC PORPHYRINS; CIRCULAR-DICHROISM; HUMAN-CHROMOSOMES},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA},
   url = {http://nar.oxfordjournals.org/content/43/9/4733.full.pdf+html},
   volume = {43},
   year = {2015}
}

TY  - JOUR
ID  - 1305254
AU  - Školáková, Petra - Trantírková, Silvie - Bednářová, Klára - Fiala, Radovan - Vorlíčková, Michaela - Trantírek, Lukáš
PY  - 2015
TI  - Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA
JF  - Nucleic Acids Research
VL  - 43
IS  - 9
SP  - 4733-4745
EP  - 4733-4745
PB  - Oxford Press
SN  - 03051048
KW  - NUCLEASE HYPERSENSITIVE ELEMENT
KW  - G-QUADRUPLEX STRUCTURES
KW  - I-MOTIF
KW  - CAENORHABDITIS-ELEGANS
KW  - HYDROGEN-BONDS
KW  - CELL-LINES
KW  - BASE-PAIRS
KW  - CATIONIC PORPHYRINS
KW  - CIRCULAR-DICHROISM
KW  - HUMAN-CHROMOSOMES
UR  - http://nar.oxfordjournals.org/content/43/9/4733.full.pdf+html
L2  - http://nar.oxfordjournals.org/content/43/9/4733.full.pdf+html
N2  - There are two basic mechanisms that are associated with the maintenance of the telomere length, which endows cancer cells with unlimited proliferative potential. One mechanism, referred to as alternative lengthening of telomeres (ALT), accounts for approximately 10-15% of all human cancers. Tumours engaged in the ALT pathway are characterised by the presence of the single stranded 5'-C-rich telomeric overhang (C-overhang). This recently identified hallmark of ALT cancers distinguishes them from healthy tissues and renders the C-overhang as a clear target for anticancer therapy. We analysed structures of the 5'-C-rich and 3'-G-rich telomeric overhangs from human and Caenorhabditis elegans, the recently established multicellular in vivo model of ALT tumours. We show that the telomeric DNA from C. elegans and humans forms fundamentally different secondary structures. The unique structural characteristics of C. elegans telomeric DNA that are distinct not only from those of humans but also from those of other multicellular eukaryotes allowed us to identify evolutionarily conserved properties of telomeric DNA. Differences in structural organisation of the telomeric DNA between the C. elegans and human impose limitations on the use of the C. elegans as an ALT tumour model.
ER  -

ŠKOLÁKOVÁ, Petra, Silvie TRANTÍRKOVÁ, Klára BEDNÁŘOVÁ, Radovan FIALA, Michaela VORLÍČKOVÁ a Lukáš TRANTÍREK. Unique C. elegans telomeric overhang structures reveal the evolutionarily conserved properties of telomeric DNA. \textit{Nucleic Acids Research}. Oxford, UK: Oxford Press, 2015, roč.~43, č.~9, s.~4733-4745. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv296.

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