Label-free bead-based metallothionein electrochemical
immunosensor

J 2015

Label-free bead-based metallothionein electrochemical immunosensor

NEJDL, Lukas, Hoai Viet NGUYEN, Lukáš RICHTERA, Soňa KŘÍŽKOVÁ, Roman GURÁŇ et. al.

Základní údaje

Originální název

Label-free bead-based metallothionein electrochemical immunosensor

Autoři

NEJDL, Lukas (203 Česká republika), Hoai Viet NGUYEN (203 Česká republika), Lukáš RICHTERA (203 Česká republika), Soňa KŘÍŽKOVÁ (203 Česká republika), Roman GURÁŇ (203 Česká republika), Michal MASAŘÍK (203 Česká republika, garant, domácí), David HYNEK (203 Česká republika), Zbynek HEGER (203 Česká republika), Karin LUNDBERG (752 Švédsko), Kristofer ERIKSON (752 Švédsko), Vojtěch ADAM (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

Electrophoresis, Hoboken, Wiley-Blackwell, 2015, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.482

Kód RIV

RIV/00216224:14110/15:00087442

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/elps.201500069

UT WoS

000360055300014

Klíčová slova anglicky

Immunosensor; Label-free; Metallothionein; Superparamagnetic particles

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 10. 2015 08:47, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 mikroL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 mikrog/mL) in real samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.

Návaznosti

NT14337, projekt VaV

Název: Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Investor: Ministerstvo zdravotnictví ČR, Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Citovat

NEJDL, Lukas, Hoai Viet NGUYEN, Lukáš RICHTERA, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Michal MASAŘÍK, David HYNEK, Zbynek HEGER, Karin LUNDBERG, Kristofer ERIKSON, Vojtěch ADAM a René KIZEK. Label-free bead-based metallothionein electrochemical immunosensor. Electrophoresis. Hoboken: Wiley-Blackwell, 2015, roč. 36, č. 16, s. 1894-1904. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201500069.

@article{1305859,
   author = {Nejdl, Lukas and Nguyen, Hoai Viet and Richtera, Lukáš and Křížková, Soňa and Guráň, Roman and Masařík, Michal and Hynek, David and Heger, Zbynek and Lundberg, Karin and Erikson, Kristofer and Adam, Vojtěch and Kizek, René},
   article_location = {Hoboken},
   article_number = {16},
   doi = {http://dx.doi.org/10.1002/elps.201500069},
   keywords = {Immunosensor; Label-free; Metallothionein; Superparamagnetic particles},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Label-free bead-based metallothionein electrochemical immunosensor},
   url = {http://dx.doi.org/10.1002/elps.201500069},
   volume = {36},
   year = {2015}
}

TY  - JOUR
ID  - 1305859
AU  - Nejdl, Lukas - Nguyen, Hoai Viet - Richtera, Lukáš - Křížková, Soňa - Guráň, Roman - Masařík, Michal - Hynek, David - Heger, Zbynek - Lundberg, Karin - Erikson, Kristofer - Adam, Vojtěch - Kizek, René
PY  - 2015
TI  - Label-free bead-based metallothionein electrochemical immunosensor
JF  - Electrophoresis
VL  - 36
IS  - 16
SP  - 1894-1904
EP  - 1894-1904
PB  - Wiley-Blackwell
SN  - 01730835
KW  - Immunosensor
KW  - Label-free
KW  - Metallothionein
KW  - Superparamagnetic particles
UR  - http://dx.doi.org/10.1002/elps.201500069
N2  - A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 mikroL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 mikrog/mL) in real samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.
ER  -

NEJDL, Lukas, Hoai Viet NGUYEN, Lukáš RICHTERA, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Michal MASAŘÍK, David HYNEK, Zbynek HEGER, Karin LUNDBERG, Kristofer ERIKSON, Vojtěch ADAM a René KIZEK. Label-free bead-based metallothionein electrochemical immunosensor. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2015, roč.~36, č.~16, s.~1894-1904. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201500069.

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