| HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Jakub BERKA, Jana HURYCHOVÁ, Jaroslav HAVLIK, Martin KAMLER, Zuzana HRONCOVÁ, Jiří KILLER, Jan TYL and Dalibor TITĚRA. Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection. In 15th Meeting of the IOBCWPRS Working Group "Microbial and Nematode Control of Invertebrathe Pests". 2015. |
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@proceedings{1306290,
author = {Hyršl, Pavel and Dobeš, Pavel and Vojtek, Libor and Berka, Jakub and Hurychová, Jana and Havlik, Jaroslav and Kamler, Martin and Hroncová, Zuzana and Killer, Jiří and Tyl, Jan and Titěra, Dalibor},
booktitle = {15th Meeting of the IOBCWPRS Working Group "Microbial and Nematode Control of Invertebrathe Pests"},
keywords = {entomopathogenic nematodes; honeybees; immunity; probiotics},
language = {eng},
title = {Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection},
year = {2015}
}
TY - CONF
ID - 1306290
AU - Hyršl, Pavel - Dobeš, Pavel - Vojtek, Libor - Berka, Jakub - Hurychová, Jana - Havlik, Jaroslav - Kamler, Martin - Hroncová, Zuzana - Killer, Jiří - Tyl, Jan - Titěra, Dalibor
PY - 2015
TI - Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection
KW - entomopathogenic nematodes
KW - honeybees
KW - immunity
KW - probiotics
N2 - Entomopathogenic nematodes (EPNs) are obligate insect parasites that are symbiotically associated with specific entomopathogenic bacteria. These nematobacterial complexes are highly pathogenic to insect hosts and therefore used in biological control of insect pests. As was shown previously, EPNs can be used also as a natural infection model and a powerful tool to study insect immunity. In this study we show that also honeybee larvae are suitable hosts for nematobacterial complex that offer an excellent environment for successful development of both nematodes and their symbiotic bacteria. Here we used EPNs for evaluation of the overall immune resistance of honeybee larvae treated with potentially immuno-modulating substances (plant alcaloid sanguinarin and probiotics). Honeybee larvae were infected with 10 IJ of Heterorhabditis bacteriophora or Steinernema feltiae per larva, both carrying their symbiotic bacteria, and then scored for mortality caused by the nematobacterial infection. In comparison to untreated honeybee larvae we observed the decrease in mortality of approx. 35 % in case of H. bacteriophora and approx. 10% upon S. feltiae infection in larvae treated by selected substances. Both sanguinarin and probiotic treatment showed similar protective effect that was less significant in highly virulent S. feltiae. This is the first record that the nematobacterial infection was used for evaluation of immune status of beneficial insect. We suggest and propose this method as a valuable tool for immunity testing in honeybees as well as in other insects. Our research is supported by grant from the Ministry of Agriculture of Czech Republic (project No. QJ1210047) and by the program CZ.1.07/2.3.00/30.0009 co-financed from European Social Fund and the state budget of the Czech Republic.
ER -
HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Jakub BERKA, Jana HURYCHOVÁ, Jaroslav HAVLIK, Martin KAMLER, Zuzana HRONCOVÁ, Jiří KILLER, Jan TYL and Dalibor TITĚRA. Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection. In \textit{15th Meeting of the IOBCWPRS Working Group ''Microbial and Nematode Control of Invertebrathe Pests''}. 2015.
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