NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC
ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC
FIBROSIS

D 2014

NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS

ĎURČ, Pavol, Michal GREGUŠ, Júlia LAČNÁ, Eva POKOJOVÁ, Jana SKŘIČKOVÁ et. al.

Základní údaje

Originální název

NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS

Autoři

ĎURČ, Pavol (703 Slovensko, domácí), Michal GREGUŠ (703 Slovensko, domácí), Júlia LAČNÁ (703 Slovensko, domácí), Eva POKOJOVÁ (203 Česká republika, domácí), Jana SKŘIČKOVÁ (203 Česká republika, domácí), František FORET (203 Česká republika, domácí) a Petr KUBÁŇ (203 Česká republika, domácí)

Vydání

BRNO, CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, od s. 182-186, 5 s. 2014

Nakladatel

INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Kód RIV

RIV/00216224:14310/14:00083393

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-904959-2-0

UT WoS

000354547400061

Klíčová slova anglicky

Capillary electrophoresis

Štítky

AKR, rivok

Změněno: 26. 4. 2016 10:06, Ing. Andrea Mikešková

Anotace

V originále

Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group of healthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably.

Citovat

ĎURČ, Pavol, Michal GREGUŠ, Júlia LAČNÁ, Eva POKOJOVÁ, Jana SKŘIČKOVÁ, František FORET a Petr KUBÁŇ. NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, s. 182-186. ISBN 978-80-904959-2-0.

@inproceedings{1306294,
   author = {Ďurč, Pavol and Greguš, Michal and Lačná, Júlia and Pokojová, Eva and Skřičková, Jana and Foret, František and Kubáň, Petr},
   address = {BRNO},
   booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K},
   keywords = {Capillary electrophoresis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {BRNO},
   isbn = {978-80-904959-2-0},
   pages = {182-186},
   publisher = {INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC},
   title = {NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS},
   year = {2014}
}

TY  - CONF
ID  - 1306294
AU  - Ďurč, Pavol - Greguš, Michal - Lačná, Júlia - Pokojová, Eva - Skřičková, Jana - Foret, František - Kubáň, Petr
PY  - 2014
TI  - NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS
PB  - INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
CY  - BRNO
SN  - 9788090495920
KW  - Capillary electrophoresis
N2  - Capillary electrophoresis (CE) with double opposite end injection (DOEI) and contactless conductivity detection (C4D) was used in a novel approach for diagnosis of cystic fibrosis (CF). A simple, fast and inexpensive "skin wipe" technique for sweat sampling was developed as a time-saving substitute for conventional, iontophoretic sweat sampling. Saliva sample was also obtained. A number of major target analytes (inorganic/organic anions and inorganic cations) were quantified simultaneously in these samples. By applying principal component analysis (PCA) to ion concentration ratios, rather than to individual ion concentrations, more accurate diagnostic tool for CF was obtained. We demonstrate that by using the developed approach and comparing a group of healthy individuals to patients diagnosed with CF, two clearly distinguished clusters can be observed without any data overlap. This approach may thus set a basis for a new method to diagnose CF more reliably.
ER  -

ĎURČ, Pavol, Michal GREGUŠ, Júlia LAČNÁ, Eva POKOJOVÁ, Jana SKŘIČKOVÁ, František FORET a Petr KUBÁŇ. NON-INVASIVE SAMPLING AND SUBSEQUENT CAPILLARY ELECTROPHORETIC ANALYSIS OF SWEAT AND SALIVA IN CLINICAL DIAGNOSIS OF CYSTIC FIBROSIS. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, s.~182-186. ISBN~978-80-904959-2-0.

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