Monitoring the ionic content of exhaled breath condensate in
various respiratory diseases by capillary electrophoresis with
contactless conductivity detection

J 2015

Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

GREGUŠ, Michal, František FORET, Dagmar KINDLOVÁ, Eva POKOJOVÁ, Marek PLUTINSKÝ et. al.

Základní údaje

Originální název

Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

Autoři

GREGUŠ, Michal (703 Slovensko, domácí), František FORET (203 Česká republika, domácí), Dagmar KINDLOVÁ (203 Česká republika), Eva POKOJOVÁ (203 Česká republika), Marek PLUTINSKÝ (703 Slovensko, domácí), Martina DOUBKOVÁ (203 Česká republika, domácí), Zdeněk MERTA (203 Česká republika, domácí), Ilona BINKOVÁ (203 Česká republika), Jana SKŘIČKOVÁ (203 Česká republika, domácí) a Petr KUBÁŇ (203 Česká republika, garant, domácí)

Vydání

JOURNAL OF BREATH RESEARCH, BRISTOL, IOP PUBLISHING LTD, 2015, 1752-7155

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.177

Kód RIV

RIV/00216224:14740/15:00080937

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1088/1752-7155/9/2/027107

UT WoS

000355656200015

Klíčová slova anglicky

exhaled breath condensate; capillary electrophoresis; contactless conductivity detection; anions and cations; respiratory diseases

Štítky

EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2016 11:45, Olga Křížová

Anotace

V originále

The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino) ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD < 1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-21919S, projekt VaV

Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu

Přiložené soubory

ZVV_1307054_Monitoring_the_ionic.pdf

Požádat o autorskou verzi souboru

Citovat

GREGUŠ, Michal, František FORET, Dagmar KINDLOVÁ, Eva POKOJOVÁ, Marek PLUTINSKÝ, Martina DOUBKOVÁ, Zdeněk MERTA, Ilona BINKOVÁ, Jana SKŘIČKOVÁ a Petr KUBÁŇ. Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection. JOURNAL OF BREATH RESEARCH. BRISTOL: IOP PUBLISHING LTD, 2015, roč. 9, č. 2, s. nestránkováno, 10 s. ISSN 1752-7155. Dostupné z: https://dx.doi.org/10.1088/1752-7155/9/2/027107.

@article{1307054,
   author = {Greguš, Michal and Foret, František and Kindlová, Dagmar and Pokojová, Eva and Plutinský, Marek and Doubková, Martina and Merta, Zdeněk and Binková, Ilona and Skřičková, Jana and Kubáň, Petr},
   article_location = {BRISTOL},
   article_number = {2},
   doi = {http://dx.doi.org/10.1088/1752-7155/9/2/027107},
   keywords = {exhaled breath condensate; capillary electrophoresis; contactless conductivity detection; anions and cations; respiratory diseases},
   language = {eng},
   issn = {1752-7155},
   journal = {JOURNAL OF BREATH RESEARCH},
   title = {Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection},
   url = {http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta},
   volume = {9},
   year = {2015}
}

TY  - JOUR
ID  - 1307054
AU  - Greguš, Michal - Foret, František - Kindlová, Dagmar - Pokojová, Eva - Plutinský, Marek - Doubková, Martina - Merta, Zdeněk - Binková, Ilona - Skřičková, Jana - Kubáň, Petr
PY  - 2015
TI  - Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
JF  - JOURNAL OF BREATH RESEARCH
VL  - 9
IS  - 2
SP  - nestránkováno
EP  - nestránkováno
PB  - IOP PUBLISHING LTD
SN  - 17527155
KW  - exhaled breath condensate
KW  - capillary electrophoresis
KW  - contactless conductivity detection
KW  - anions and cations
KW  - respiratory diseases
UR  - http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta
L2  - http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta
N2  - The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino) ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD < 1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.
ER  -

GREGUŠ, Michal, František FORET, Dagmar KINDLOVÁ, Eva POKOJOVÁ, Marek PLUTINSKÝ, Martina DOUBKOVÁ, Zdeněk MERTA, Ilona BINKOVÁ, Jana SKŘIČKOVÁ a Petr KUBÁŇ. Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection. \textit{JOURNAL OF BREATH RESEARCH}. BRISTOL: IOP PUBLISHING LTD, 2015, roč.~9, č.~2, s.~nestránkováno, 10 s. ISSN~1752-7155. Dostupné z: https://dx.doi.org/10.1088/1752-7155/9/2/027107.

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