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@article{1307096,
author = {Švec, Pavel and De Bel, Annelies and Sedláček, Ivo and Petráš, Petr and Gelbíčová, Tereza and Černohlávková, Jitka and Mašlaňová, Ivana and Varbanovová, Ivana and Cnockaert, Margo and Echahidi, Fedoua and Vandamme, Peter and Pantůček, Roman},
article_location = {London, UK},
article_number = {July},
doi = {http://dx.doi.org/10.1099/ijs.0.000220},
keywords = {coagulase-negative staphylococci; Staphylococcus petrasii subsp. pragensis; rep-PCR; molecular phylogeny; MALDI-TOF mass spectrometry; fatty acid methyl ester analysis; DNA-DNA hybridization; automated ribotyping},
language = {eng},
issn = {1466-5026},
journal = {International Journal of Systematic and Evolutionary Microbiology},
title = {Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material},
url = {http://ijs.sgmjournals.org/content/journal/ijsem/10.1099/ijs.0.000220},
volume = {65},
year = {2015}
}
TY - JOUR
ID - 1307096
AU - Švec, Pavel - De Bel, Annelies - Sedláček, Ivo - Petráš, Petr - Gelbíčová, Tereza - Černohlávková, Jitka - Mašlaňová, Ivana - Varbanovová, Ivana - Cnockaert, Margo - Echahidi, Fedoua - Vandamme, Peter - Pantůček, Roman
PY - 2015
TI - Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material
JF - International Journal of Systematic and Evolutionary Microbiology
VL - 65
IS - July
SP - 2071-2077
EP - 2071-2077
PB - Society for General Microbiology
SN - 14665026
KW - coagulase-negative staphylococci
KW - Staphylococcus petrasii subsp. pragensis
KW - rep-PCR
KW - molecular phylogeny
KW - MALDI-TOF mass spectrometry
KW - fatty acid methyl ester analysis
KW - DNA-DNA hybridization
KW - automated ribotyping
UR - http://ijs.sgmjournals.org/content/journal/ijsem/10.1099/ijs.0.000220
L2 - http://ijs.sgmjournals.org/content/journal/ijsem/10.1099/ijs.0.000220
N2 - Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA–DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356T (=CCM 8529T=LMG 28327T).
ER -
ŠVEC, Pavel, Annelies DE BEL, Ivo SEDLÁČEK, Petr PETRÁŠ, Tereza GELBÍČOVÁ, Jitka ČERNOHLÁVKOVÁ, Ivana MAŠLAŇOVÁ, Ivana VARBANOVOVÁ, Margo CNOCKAERT, Fedoua ECHAHIDI, Peter VANDAMME a Roman PANTŮČEK. Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material. \textit{International Journal of Systematic and Evolutionary Microbiology}. London, UK: Society for General Microbiology, 2015, roč.~65, July, s.~2071-2077. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.000220.
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