PERNIKÁŘOVÁ, Vendula, Vojtěch SEDLÁČEK, David POTĚŠIL, Iva PROCHÁZKOVÁ, Zbyněk ZDRÁHAL, Pavel BOUCHAL and Igor KUČERA. Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans. Data in Brief. Amsterdam: Elsevier, 2015, vol. 4, September, p. 390-394. ISSN 2352-3409. Available from: https://dx.doi.org/10.1016/j.dib.2015.06.015.
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Basic information
Original name Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans
Authors PERNIKÁŘOVÁ, Vendula (203 Czech Republic, belonging to the institution), Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Iva PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Pavel BOUCHAL (203 Czech Republic, belonging to the institution) and Igor KUČERA (203 Czech Republic, guarantor, belonging to the institution).
Edition Data in Brief, Amsterdam, Elsevier, 2015, 2352-3409.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/15:00080961
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.dib.2015.06.015
UT WoS 000453157400072
Keywords in English Paracoccus denitrificans; 3DLC proteomics; FerB; iTRAQ; Microbial proteomics
Tags AKR, CF PROT, rivok
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Igor Kučera, DrSc., učo 911. Changed: 20/7/2019 14:20.
Abstract
3DLC protein- and peptide fractionation technique combined with iTRAQ-peptide labeling and Orbitrap mass spectrometry was employed to quantitate Paracoccus dentirificans total proteome with maximal coverage. This resulted in identification of 24948 peptides representing 2627 proteins (FDR<0.01) in P. dentirificans wild type and ferB mutant strains grown in the presence or absence of methyl viologen as an oxidative stressor. The data were generated for assessment of FerB protein role in oxidative stress as published by V. Pernikářová et al., Proteomic responses to a methyl viologeninduced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans, J Proteomics 2015;125:68-75. Dataset is supplied in the article.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP503/12/0369, research and development projectName: Nové flavin-dependentní enzymy Paracoccus denitrificans: reakční mechanismy, metabolické funkce a úloha v buněčném oxidačním stresu
Investor: Czech Science Foundation
GA14-19250S, research and development projectName: Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk
Investor: Czech Science Foundation
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