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@article{1309141,
author = {Fojtová, Miloslava and Sýkorová, Eva and Najdekrová, Lucie and Polanská, Pavla and Zachová, Dagmar and Vágnerová, Radka and Angelis, Karel J. and Fajkus, Jiří},
article_location = {Springer},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s11103-015-0299-9},
keywords = {Physcomitrella patens; Telomere maintenance; Telomerase; PpTERT structure; DSB repair mutants},
language = {eng},
issn = {0167-4412},
journal = {Plant Molecular Biology},
title = {Telomere dynamics in the lower plant Physcomitrella patens},
url = {http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02},
volume = {87},
year = {2015}
}
TY - JOUR
ID - 1309141
AU - Fojtová, Miloslava - Sýkorová, Eva - Najdekrová, Lucie - Polanská, Pavla - Zachová, Dagmar - Vágnerová, Radka - Angelis, Karel J. - Fajkus, Jiří
PY - 2015
TI - Telomere dynamics in the lower plant Physcomitrella patens
JF - Plant Molecular Biology
VL - 87
IS - 6
SP - 591-601
EP - 591-601
PB - Dordrecht (Netherlands)
SN - 01674412
KW - Physcomitrella patens
KW - Telomere maintenance
KW - Telomerase
KW - PpTERT structure
KW - DSB repair mutants
UR - http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02
L2 - http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02
N2 - A comparative approach in biology is needed to assess the universality of rules governing this discipline. In plant telomere research, most of the key principles were established based on studies in only single model plant, Arabidopsis thaliana. These principles include the absence of telomere shortening during plant development and the corresponding activity of telomerase in dividing (meristem) plant cells. Here we examine these principles in Physcomitrella patens as a representative of lower plants. To follow telomerase expression, we first characterize the gene coding for the telomerase reverse transcriptase subunit PpTERT in P. patens, for which only incomplete prediction has been available so far. In protonema cultures of P. patens, growing by filament apical cell division, the proportion of apical (dividing) cells was quantified and telomere length, telomerase expression and activity were determined. Our results show telomere stability and demonstrate proportionality of telomerase activity and expression with the number of apical cells. In addition, we analyze telomere maintenance in mre11, rad50, nbs1, ku70 and lig4 mutants of P. patens and compare the impact of these mutations in double-strand-break (DSB) repair pathways with earlier observations in corresponding A. thaliana mutants. Telomere phenotypes are absent and DSB repair kinetics is not affected in P. patens mutants for DSB factors involved in non-homologous end joining (NHEJ). This is compliant with the overall dominance of homologous recombination over NHEJ pathways in the moss, contrary to the inverse situation in flowering plants.
ER -
FOJTOVÁ, Miloslava, Eva SÝKOROVÁ, Lucie NAJDEKROVÁ, Pavla POLANSKÁ, Dagmar ZACHOVÁ, Radka VÁGNEROVÁ, Karel J. ANGELIS a Jiří FAJKUS. Telomere dynamics in the lower plant Physcomitrella patens. \textit{Plant Molecular Biology}. Springer: Dordrecht (Netherlands), 2015, roč.~87, č.~6, s.~591-601. ISSN~0167-4412. Dostupné z: https://dx.doi.org/10.1007/s11103-015-0299-9.
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