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@misc{1309240,
author = {Pernisová, Markéta and Hejátko, Jan},
booktitle = {6th Methods in Plant Science},
keywords = {microscopy; plants},
language = {eng},
title = {In-vivo Fluorescence Macroscopy in Plants},
year = {2014}
}
TY - SLIDE
ID - 1309240
AU - Pernisová, Markéta - Hejátko, Jan
PY - 2014
TI - In-vivo Fluorescence Macroscopy in Plants
KW - microscopy
KW - plants
N2 - Many microscopic techniques exist in plant biology and microscopic slides are necessary to use usually. Together with Nikon company we developed horizontally oriented confocal macroscope for in-vivo plant imaging on plates directly. Optical system with objectives is horizontally oriented and thus plants can grow vertically similarly to real conditions in soil. This setup allows observing plant’s biological processes in a real time, for example protein relocalization, changes in metabolite concentration, cell elongation and division or root growth. No transmitted light is a small disadvantage but it is possible to use plant lines with fluorescent protein labelled plasma membranes. Taken together, horizontal confocal macroscope is very useful instrument for real-time imaging of growing plants.
ER -
PERNISOVÁ, Markéta and Jan HEJÁTKO. In-vivo Fluorescence Macroscopy in Plants. In \textit{6th Methods in Plant Science}. 2014.
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