KVÁČ, Martin, Anna HOŘICKÁ, Bohumil SAK, Jitka PREDIGER, Jiří SALÁT, Jana ŠIRMAROVÁ, Tomáš BARTONIČKA, Mark CLARK, Joba Rose CHELLADURAI, Erin GILLAM and John MCEVOY. Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic. Parasitology Research. 2015, vol. 114, No 10, p. 3917-3921. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4654-1.
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Basic information
Original name Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic
Authors KVÁČ, Martin (203 Czech Republic, guarantor), Anna HOŘICKÁ (203 Czech Republic), Bohumil SAK (203 Czech Republic), Jitka PREDIGER (203 Czech Republic), Jiří SALÁT (203 Czech Republic), Jana ŠIRMAROVÁ (203 Czech Republic), Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution), Mark CLARK (840 United States of America), Joba Rose CHELLADURAI (840 United States of America), Erin GILLAM (840 United States of America) and John MCEVOY (840 United States of America).
Edition Parasitology Research, 2015, 0932-0113.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.027
RIV identification code RIV/00216224:14310/15:00083668
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00436-015-4654-1
UT WoS 000360860900039
Keywords in English Cryptosporidium Bats SSU Actin PCR
Tags AKR, rivok
Changed by Changed by: doc. Mgr. Tomáš Bartonička, Ph.D., učo 54832. Changed: 19/2/2018 10:39.
Abstract
Bats from the families Rhinolophidae (n=90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium oocysts were not detected in any of the 281 faecal specimens examined using the aniline–carbol– methyl violet staining method. Nested PCR amplification, sequencing and phylogenetic analysis of the small ribosomal subunit rRNA and actin genes were used to identify isolates and infer evolutionary relationships. Cryptosporidium parvum was identified in a western small-footed bat (Myotis ciliolabrum) from the USA and a common pipistrelle bats (Pipistrellus pipistrellus) from the Czech Republic. Two novel genotypes were identified and named Cryptosporidium bat genotype III and IV. Bat genotype III was found in two big brown bats (Eptesicus fuscus) from the USA. Bat genotype IV was detected in two common pipistrelle bats from the Czech Republic.
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