J 2015

Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic

KVÁČ, Martin, Anna HOŘICKÁ, Bohumil SAK, Jitka PREDIGER, Jiří SALÁT et. al.

Basic information

Original name

Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic

Authors

KVÁČ, Martin (203 Czech Republic, guarantor), Anna HOŘICKÁ (203 Czech Republic), Bohumil SAK (203 Czech Republic), Jitka PREDIGER (203 Czech Republic), Jiří SALÁT (203 Czech Republic), Jana ŠIRMAROVÁ (203 Czech Republic), Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution), Mark CLARK (840 United States of America), Joba Rose CHELLADURAI (840 United States of America), Erin GILLAM (840 United States of America) and John MCEVOY (840 United States of America)

Edition

Parasitology Research, 2015, 0932-0113

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.027

RIV identification code

RIV/00216224:14310/15:00083668

Organization unit

Faculty of Science

UT WoS

000360860900039

Keywords in English

Cryptosporidium Bats SSU Actin PCR

Tags

Změněno: 19/2/2018 10:39, doc. Mgr. Tomáš Bartonička, Ph.D.

Abstract

V originále

Bats from the families Rhinolophidae (n=90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium oocysts were not detected in any of the 281 faecal specimens examined using the aniline–carbol– methyl violet staining method. Nested PCR amplification, sequencing and phylogenetic analysis of the small ribosomal subunit rRNA and actin genes were used to identify isolates and infer evolutionary relationships. Cryptosporidium parvum was identified in a western small-footed bat (Myotis ciliolabrum) from the USA and a common pipistrelle bats (Pipistrellus pipistrellus) from the Czech Republic. Two novel genotypes were identified and named Cryptosporidium bat genotype III and IV. Bat genotype III was found in two big brown bats (Eptesicus fuscus) from the USA. Bat genotype IV was detected in two common pipistrelle bats from the Czech Republic.