Complete genome analysis of ETA-converting bacteriophages
isolated from impetigo strains of Staphylococcus aureus

D 2015

Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK et. al.

Základní údaje

Originální název

Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus

Autoři

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK, Zbyněk ZDRÁHAL, Petr PETRÁŠ a Jiří DOŠKAŘ

Vydání

1. vyd. Brno, Týden lékařské genetiky v Brně - The Biomania Student Scientific Meeting 2015, s. 33-33, 2015

Nakladatel

Masarykova univerzita, Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

The Biomania Student Scientific Meeting 2015

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-7933-5

Klíčová slova anglicky

ETA phage, Exfoliative toxin A, structure proteins spectre, genome analysis

Štítky

Příznaky

Recenzováno

Změněno: 10. 5. 2018 09:35, Ing. Nicole Zrilić

Anotace

V originále

Exfoliative toxin A (ETA)-coding temperate bacteriophages are the main contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two eta gene-positive phages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in several Czech maternity hospitals were characterized in detail to elucidate their genome diversity. The phages, designated phiB166 and phiB236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined and described. In addition, promoters and intrinsic terminators were predicted. With respect to the functional genomic architecture, the genomes were divided into five regions. The comparative analysis revealed major variances between the phages. They differed in the genome size, number of open reading frames (ORFs) and genome architecture. High mutual sequence similarity was detected only in the terminal region. Proteomic analysis showed differences in virion protein patterns. When compared with the so far described eta and non eta phages, noticeable differences were found. Sequences gained by possible recombination were identified in genomes of both phages. Thus, these phages represent two new types of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

NT12395, projekt VaV

Název: Molekulární průkaz a analýza invazivních kmenů small colony variants (SCV) a rezistentních kmenů S. aureus od pacientů s cystickou fibrózou

Citovat

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK, Zbyněk ZDRÁHAL, Petr PETRÁŠ a Jiří DOŠKAŘ. Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus. In R. Gaillyová, I. Valášková, M. Jarkovská. Týden lékařské genetiky v Brně - The Biomania Student Scientific Meeting 2015. 1. vyd. Brno: Masarykova univerzita, Brno, 2015, s. 33. ISBN 978-80-210-7933-5.

@inproceedings{1311990,
   author = {Botka, Tibor and Růžičková, Vladislava and Konečná, Hana and Pantůček, Roman and Rychlík, Ivan and Zdráhal, Zbyněk and Petráš, Petr and Doškař, Jiří},
   address = {Brno},
   booktitle = {Týden lékařské genetiky v Brně - The Biomania Student Scientific Meeting 2015},
   edition = {1.},
   editor = {R. Gaillyová, I. Valášková, M. Jarkovská},
   keywords = {ETA phage, Exfoliative toxin A, structure proteins spectre, genome analysis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-7933-5},
   note = {Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+},
   pages = {33-33},
   publisher = {Masarykova univerzita, Brno},
   title = {Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus},
   url = {http://www.biomania.cz/en/events-and-actions/biomania-student-scientific-meeting-2015/},
   year = {2015}
}

TY  - JOUR
ID  - 1311990
AU  - Botka, Tibor - Růžičková, Vladislava - Konečná, Hana - Pantůček, Roman - Rychlík, Ivan - Zdráhal, Zbyněk - Petráš, Petr - Doškař, Jiří
PY  - 2015
TI  - Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus
PB  - Masarykova univerzita, Brno
CY  - Brno
SN  - 9788021079335
N1  - Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+
KW  - ETA phage, Exfoliative toxin A, structure proteins spectre, genome analysis
UR  - http://www.biomania.cz/en/events-and-actions/biomania-student-scientific-meeting-2015/
N2  - Exfoliative toxin A (ETA)-coding temperate bacteriophages are the main contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two eta gene-positive phages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in several Czech maternity hospitals were characterized in detail to elucidate their genome diversity. The phages, designated phiB166 and phiB236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined and described. In addition, promoters and intrinsic terminators were predicted. With respect to the functional genomic architecture, the genomes were divided into five regions. The comparative analysis revealed major variances between the phages. They differed in the genome size, number of open reading frames (ORFs) and genome architecture. High mutual sequence similarity was detected only in the terminal region. Proteomic analysis showed differences in virion protein patterns. When compared with the so far described eta and non eta phages, noticeable differences were found. Sequences gained by possible recombination were identified in genomes of both phages. Thus, these phages represent two new types of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.
ER  -

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK, Zbyněk ZDRÁHAL, Petr PETRÁŠ a Jiří DOŠKAŘ. Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus. In R. Gaillyová, I. Valášková, M. Jarkovská. \textit{Týden lékařské genetiky v Brně - The Biomania Student Scientific Meeting 2015}. 1. vyd. Brno: Masarykova univerzita, Brno, 2015, s.~33. ISBN~978-80-210-7933-5.

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