Contributions of the TEL-patch Amino Acid Cluster on TPP1 to
Telomeric DNA Synthesis by Human Telomerase

J 2015

Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase

DALBY, AB, Ctirad HOFR a TR CECH

Základní údaje

Originální název

Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase

Autoři

DALBY, AB (840 Spojené státy), Ctirad HOFR (203 Česká republika, garant, domácí) a TR CECH (840 Spojené státy)

Vydání

Journal of Molecular Biology, LONDON, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2015, 0022-2836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.517

Kód RIV

RIV/00216224:14310/15:00081074

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jmb.2015.01.008

UT WoS

000351798700006

Klíčová slova anglicky

telomere; telomerase recruitment; DNA substrate-competition; TPP1 TEL-patch; processivity

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2016 16:08, Ing. Andrea Mikešková

Anotace

V originále

Telomere maintenance is a highly coordinated process, and its misregulation is linked to cancer and telomere-shortening syndromes. Recent studies have shown that the TEL-patch-a cluster of amino acids on the surface of the shelterin component TPP1-is necessary for the recruitment of telonnerase to the telomere in human cells. However, there has been only basic biochemical analysis of the role of TPP1 in the telomerase recruitment process. Here we develop an in vitro assay to quantitatively measure the contribution of the TEL-patch to telomerase recruitment-binding and extension of the first telomeric repeat. We also demonstrate that the TEL-patch contributes to the translocation step of the telomerase reaction. Finally, our quantitative observations indicate that the TEL-patch stabilizes the association between telomerase and telomeric DNA substrates, providing a molecular explanation for its contributions to telomerase recruitment and action. (C) 2015 Elsevier Ltd. All rights reserved.

Návaznosti

GAP205/12/0550, projekt VaV

Název: Dynamika vzniku shelterinového komplexu

Investor: Grantová agentura ČR, Dynamika vzniku shelterinového komplexu

LH13054, projekt VaV

Název: Studium dynamiky telomerových proteinových komplexů zajišťujících stabilitu genomu (Akronym: TeloProtGen)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Studium dynamiky telomerových proteinových komplexů zajišťujících stabilitu genomu

Citovat

DALBY, AB, Ctirad HOFR a TR CECH. Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase. Journal of Molecular Biology. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2015, roč. 427, č. 6, s. 1291-1303. ISSN 0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2015.01.008.

@article{1312758,
   author = {Dalby, AB and Hofr, Ctirad and Cech, TR},
   article_location = {LONDON},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.jmb.2015.01.008},
   keywords = {telomere; telomerase recruitment; DNA substrate-competition; TPP1 TEL-patch; processivity},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase},
   url = {http://www.sciencedirect.com/science/article/pii/S0022283615000297},
   volume = {427},
   year = {2015}
}

TY  - JOUR
ID  - 1312758
AU  - Dalby, AB - Hofr, Ctirad - Cech, TR
PY  - 2015
TI  - Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase
JF  - Journal of Molecular Biology
VL  - 427
IS  - 6
SP  - 1291-1303
EP  - 1291-1303
PB  - ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
SN  - 00222836
KW  - telomere
KW  - telomerase recruitment
KW  - DNA substrate-competition
KW  - TPP1 TEL-patch
KW  - processivity
UR  - http://www.sciencedirect.com/science/article/pii/S0022283615000297
N2  - Telomere maintenance is a highly coordinated process, and its misregulation is linked to cancer and telomere-shortening syndromes. Recent studies have shown that the TEL-patch-a cluster of amino acids on the surface of the shelterin component TPP1-is necessary for the recruitment of telonnerase to the telomere in human cells. However, there has been only basic biochemical analysis of the role of TPP1 in the telomerase recruitment process. Here we develop an in vitro assay to quantitatively measure the contribution of the TEL-patch to telomerase recruitment-binding and extension of the first telomeric repeat. We also demonstrate that the TEL-patch contributes to the translocation step of the telomerase reaction. Finally, our quantitative observations indicate that the TEL-patch stabilizes the association between telomerase and telomeric DNA substrates, providing a molecular explanation for its contributions to telomerase recruitment and action. (C) 2015 Elsevier Ltd. All rights reserved.
ER  -

DALBY, AB, Ctirad HOFR a TR CECH. Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase. \textit{Journal of Molecular Biology}. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2015, roč.~427, č.~6, s.~1291-1303. ISSN~0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2015.01.008.

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