Atomic force microscopy for characterization of biomolecules,
affinity complexes and cells

D 2015

Atomic force microscopy for characterization of biomolecules, affinity complexes and cells

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ et. al.

Basic information

Original name

Atomic force microscopy for characterization of biomolecules, affinity complexes and cells

Authors

SKLÁDAL, Petr (203 Czech Republic, guarantor, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Veronika HORŇÁKOVÁ (203 Czech Republic, belonging to the institution), Patrik GEREG (703 Slovakia, belonging to the institution), Zdenka FOHLEROVÁ (203 Czech Republic, belonging to the institution), Martin JAKUBEC (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Martin PEŠL (203 Czech Republic, belonging to the institution)

Edition

Brno, XV. Workshop of Physical Chemists and Electrochemists, p. 38-39, 2 pp. 2015

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14740/15:00084143

Organization unit

Central European Institute of Technology

ISBN

978-80-210-7857-4

Keywords in English

atomic force microscopy; force-distance curves; biomolecular interactions

Abstract

V originále

The instrumentation and services available at the Core Facility of Nanobiotechnology in CEITEC MU will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids, their affinity complexes and cells. The interaction of ssDNA binding protein with oligonucleotides was imaged using bare tips (Fig. 1), the binding forces in the affinity complex were studied using the protein ligand-modified tip and the ForceRobot AFM head for automated recording of force-distance curves. Similar experiments characterised immunoreactions between antibody and serum albumin and microbial cells as antigens and hybridisation of nucleic acids; interactions were confirmed using surface plasmon resonance and electrochemical measurements. Properties of mast cells were characterised in relation to the changes accompanying biotransformation events (Fig. 2). Simultaneous recording of contractions and electric activity of cardiomyocytes was followed using the AFM cantilever with conductive tip functioning as nanomechanical transducer for cellular biosensor suitable for evaluation of physiologically active compounds in real time.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ, Martin JAKUBEC, Zdeněk FARKA, David KOVÁŘ and Martin PEŠL. Atomic force microscopy for characterization of biomolecules, affinity complexes and cells. Online. In Libuše Trnková. XV. Workshop of Physical Chemists and Electrochemists. Brno: Masaryk University, 2015, p. 38-39. ISBN 978-80-210-7857-4.

@inproceedings{1313155,
   author = {Skládal, Petr and Přibyl, Jan and Horňáková, Veronika and Gereg, Patrik and Fohlerová, Zdenka and Jakubec, Martin and Farka, Zdeněk and Kovář, David and Pešl, Martin},
   address = {Brno},
   booktitle = {XV. Workshop of Physical Chemists and Electrochemists},
   editor = {Libuše Trnková},
   keywords = {atomic force microscopy; force-distance curves; biomolecular interactions},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-7857-4},
   pages = {38-39},
   publisher = {Masaryk University},
   title = {Atomic force microscopy for characterization of biomolecules, affinity complexes and cells},
   url = {http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2015.pdf#page=38},
   year = {2015}
}

TY  - JOUR
ID  - 1313155
AU  - Skládal, Petr - Přibyl, Jan - Horňáková, Veronika - Gereg, Patrik - Fohlerová, Zdenka - Jakubec, Martin - Farka, Zdeněk - Kovář, David - Pešl, Martin
PY  - 2015
TI  - Atomic force microscopy for characterization of biomolecules, affinity complexes and cells
PB  - Masaryk University
CY  - Brno
SN  - 9788021078574
KW  - atomic force microscopy
KW  - force-distance curves
KW  - biomolecular interactions
UR  - http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2015.pdf#page=38
N2  - The instrumentation and services available at the Core Facility of Nanobiotechnology in CEITEC MU will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids, their affinity complexes and cells. The interaction of ssDNA binding protein with oligonucleotides was imaged using bare tips (Fig. 1), the binding forces in the affinity complex were studied using the protein ligand-modified tip and the ForceRobot AFM head for automated recording of force-distance curves. Similar experiments characterised immunoreactions between antibody and serum albumin and microbial cells as antigens and hybridisation of nucleic acids; interactions were confirmed using surface plasmon resonance and electrochemical measurements. Properties of mast cells were characterised in relation to the changes accompanying biotransformation events (Fig. 2). Simultaneous recording of contractions and electric activity of cardiomyocytes was followed using the AFM cantilever with conductive tip functioning as nanomechanical transducer for cellular biosensor suitable for evaluation of physiologically active compounds in real time.
ER  -

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ, Martin JAKUBEC, Zdeněk FARKA, David KOVÁŘ and Martin PEŠL. Atomic force microscopy for characterization of biomolecules, affinity complexes and cells. Online. In Libuše Trnková. \textit{XV. Workshop of Physical Chemists and Electrochemists}. Brno: Masaryk University, 2015, p.~38-39. ISBN~978-80-210-7857-4.

Detailed Information on Publication Record