Combination of MiR-378 and MiR-210 Serum Levels Enables
Sensitive Detection of Renal Cell Carcinoma

J 2015

Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma

FEDORKO, Michal; Michal STANÍK; Robert ILIEV; Martina LOJOVÁ; Táňa MACHÁČKOVÁ et al.

Základní údaje

Originální název

Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma

Autoři

FEDORKO, Michal; Michal STANÍK; Robert ILIEV; Martina LOJOVÁ; Táňa MACHÁČKOVÁ; Marek SVOBODA; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ

Vydání

International Journal of Molecular Sciences, Basel, MDPI AG, 2015, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00084162

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

renální karcinom; mikroRNA; krevní sérum; biomarker

Klíčová slova anglicky

renal cell carcinoma; microRNA; blood serum; biomarker

Štítky

EL OK, OA, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 4. 2016 12:55, Olga Křížová

Anotace

V originále

Serum microRNAs are emerging as a clinically useful tool for early and non-invasive detection of various cancer types including renal cell carcinoma (RCC). Based on our previous results, we performed the study to analyze circulating serum miR-378 and miR-210 in patients with various histological subtypes of RCC. RNA was purified from blood serum samples of 195 RCC patients and 100 healthy controls. The levels of miR-378 and miR-210 in serum were determined absolutely using quantitative real-time PCR. Pre- and postoperative levels of both microRNAs were compared in 20 RCC patients. Significantly increased serum levels of both miR-378 and miR-210 enabled to clearly distinguish RCC patients and healthy controls with 80% sensitivity and 78% specificity if analyzed in combination (p < 0.0001), and their levels significantly decreased in the time period of three months after radical nephrectomy (p < 0.0001). Increased level of miR-378 positively correlates with disease-free survival (p = 0.036) and clinical stage (p = 0.0476). The analysis of serum miR-378 and miR-210 proved their potential to serve as powerful non-invasive diagnostic and prognostic biomarkers in RCC.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

NV15-34678A, projekt VaV

Název: Molekulární prognostické a prediktivní faktory u pacientů s metastatickým renálním karcinomem léčených tyrozinkinázovými inhibitory

Přiložené soubory

ZVV_2015_1313221_Combination_of_MiR-378.pdf

Požádat o autorskou verzi souboru

EL_1313221.pdf

Požádat o autorskou verzi souboru

1313221_Combination.pdf

Požádat o autorskou verzi souboru

Citovat

FEDORKO, Michal; Michal STANÍK; Robert ILIEV; Martina LOJOVÁ; Táňa MACHÁČKOVÁ; Marek SVOBODA; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ. Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma. International Journal of Molecular Sciences. Basel: MDPI AG, 2015, roč. 16, č. 10, s. 23382-23389. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms161023382.

@article{1313221,
   author = {Fedorko, Michal and Staník, Michal and Iliev, Robert and Lojová, Martina and Macháčková, Táňa and Svoboda, Marek and Pacík, Dalibor and Doležel, Jan and Slabý, Ondřej},
   article_location = {Basel},
   article_number = {10},
   doi = {https://doi.org/10.3390/ijms161023382},
   keywords = {renal cell carcinoma; microRNA; blood serum; biomarker},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma},
   url = {http://www.mdpi.com/1422-0067/16/10/23382},
   volume = {16},
   year = {2015}
}

TY  - JOUR
ID  - 1313221
AU  - Fedorko, Michal - Staník, Michal - Iliev, Robert - Lojová, Martina - Macháčková, Táňa - Svoboda, Marek - Pacík, Dalibor - Doležel, Jan - Slabý, Ondřej
PY  - 2015
TI  - Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma
JF  - International Journal of Molecular Sciences
VL  - 16
IS  - 10
SP  - 23382-23389
EP  - 23382-23389
PB  - MDPI AG
SN  - 14220067
KW  - renal cell carcinoma
KW  - microRNA
KW  - blood serum
KW  - biomarker
UR  - http://www.mdpi.com/1422-0067/16/10/23382
L2  - http://www.mdpi.com/1422-0067/16/10/23382
N2  - Serum microRNAs are emerging as a clinically useful tool for early and non-invasive detection of various cancer types including renal cell carcinoma (RCC). Based on our previous results, we performed the study to analyze circulating serum miR-378 and miR-210 in patients with various histological subtypes of RCC. RNA was purified from blood serum samples of 195 RCC patients and 100 healthy controls. The levels of miR-378 and miR-210 in serum were determined absolutely using quantitative real-time PCR. Pre- and postoperative levels of both microRNAs were compared in 20 RCC patients. Significantly increased serum levels of both miR-378 and miR-210 enabled to clearly distinguish RCC patients and healthy controls with 80% sensitivity and 78% specificity if analyzed in combination (p < 0.0001), and their levels significantly decreased in the time period of three months after radical nephrectomy (p < 0.0001). Increased level of miR-378 positively correlates with disease-free survival (p = 0.036) and clinical stage (p = 0.0476). The analysis of serum miR-378 and miR-210 proved their potential to serve as powerful non-invasive diagnostic and prognostic biomarkers in RCC.
ER  -

FEDORKO, Michal; Michal STANÍK; Robert ILIEV; Martina LOJOVÁ; Táňa MACHÁČKOVÁ; Marek SVOBODA; Dalibor PACÍK; Jan DOLEŽEL a Ondřej SLABÝ. Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma. \textit{International Journal of Molecular Sciences}. Basel: MDPI AG, 2015, roč.~16, č.~10, s.~23382-23389. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms161023382.

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