| VÁVROVÁ, Andrea, Tereza BALÁŽOVÁ, Ivo SEDLÁČEK, Ludmila TVRZOVÁ a Ondrej ŠEDO. Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp. Folia Microbiologica. AV ČR, Institute of Microbiology, 2015, roč. 60, č. 5, s. 375-383. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-014-0369-4. |
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@article{1313800,
author = {Vávrová, Andrea and Balážová, Tereza and Sedláček, Ivo and Tvrzová, Ludmila and Šedo, Ondrej},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s12223-014-0369-4},
keywords = {DESORPTION/IONIZATION-MASS SPECTROMETRY; GENUS AEROMONAS; SP-NOV.; PHYLOGENETIC ANALYSIS; HYDROPHILA; AQUARIORUM; WATER; ENTEROPELOGENES; BACTERIA; STRAINS},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.},
volume = {60},
year = {2015}
}
TY - JOUR
ID - 1313800
AU - Vávrová, Andrea - Balážová, Tereza - Sedláček, Ivo - Tvrzová, Ludmila - Šedo, Ondrej
PY - 2015
TI - Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
JF - Folia Microbiologica
VL - 60
IS - 5
SP - 375-383
EP - 375-383
PB - AV ČR, Institute of Microbiology
SN - 00155632
KW - DESORPTION/IONIZATION-MASS SPECTROMETRY
KW - GENUS AEROMONAS
KW - SP-NOV.
KW - PHYLOGENETIC ANALYSIS
KW - HYDROPHILA
KW - AQUARIORUM
KW - WATER
KW - ENTEROPELOGENES
KW - BACTERIA
KW - STRAINS
N2 - The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.
ER -
VÁVROVÁ, Andrea, Tereza BALÁŽOVÁ, Ivo SEDLÁČEK, Ludmila TVRZOVÁ a Ondrej ŠEDO. Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp. \textit{Folia Microbiologica}. AV ČR, Institute of Microbiology, 2015, roč.~60, č.~5, s.~375-383. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-014-0369-4.
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