2016
Olfactory bulbectomy increases reinstatement of methamphetamine seeking after a forced abstinence in rats
BABINSKÁ, Zuzana; Jana RUDÁ; Petra AMCHOVÁ; Jana MERHAUTOVÁ; Ladislav DUŠEK et al.Základní údaje
Originální název
Olfactory bulbectomy increases reinstatement of methamphetamine seeking after a forced abstinence in rats
Název česky
Olfaktorická bulbektomie zvyšuje chování vedoucí k vyhledávání metamfetaminu po období nucené abstinence u potkana
Autoři
BABINSKÁ, Zuzana; Jana RUDÁ; Petra AMCHOVÁ; Jana MERHAUTOVÁ ORCID; Ladislav DUŠEK a Alexandra ŠULCOVÁ
Vydání
Behavioural Brain Research, Amsterdam, Elsevier Science BV, 2016, 0166-4328
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30104 Pharmacology and pharmacy
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.002
Kód RIV
RIV/00216224:14110/16:00089131
Organizační jednotka
Lékařská fakulta
UT WoS
000367107900004
EID Scopus
2-s2.0-84944145064
Klíčová slova anglicky
Sprague-Dawley rats; depression; methamphetamine; olfactory bulbectomy; reinstatement; self-administration
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 20. 6. 2022 12:00, Mgr. Tereza Miškechová
Anotace
V originále
Drug addiction is commonly associated with depression and comorbid patients also suffer from higher cravings and increased relapse rate. To address this issue preclinically we combined the olfactory bulbectomy (OBX) model of depression and intravenous methamphetamine self-administration procedure in rats to assess differences in relapse-like behavior. Male Sprague-Dawley rats were divided randomly into two groups; in one group the bilateral olfactory bulbectomy (OBX) was performed while the other group was sham operated. After recovery, intracardiac catheter was implanted. Intravenous self-administration procedure was conducted in operant boxes using nose-poke operandi (Coulbourn Instruments, Inc., USA) under fixed ratio 1 schedule of reinforcement. Methamphetamine was available at dose 0.08 mg/kg/infusion. After stable methamphetamine intake was maintained, a period of forced abstinence was initiated and rats were kept in their home-cages for 14 days. Finally, one reinstatement session was conducted in operant boxes with no drug delivery. In the reinstatement session the mean of 138.4 active nose-pokes was performed by the OBX group, while the sham group displayed 41 responses, i.e. 140 % and 48 % of basal nose-poking during maintenance phase in OBX and sham operated group respectively. OBX group also showed significantly more passive nose-pokes indicating hyperactive behavioral traits in bulbectomized rats. However, the % of active operandum preference was equal in both groups. Olfactory bulbectomy model significantly increased reinstatement of methamphetamine seeking behavior. This paradigm can be used to evaluate potential drugs that are able to suppress the drug-seeking behavior.
Návaznosti
| ED1.1.00/02.0068, projekt VaV |
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| LM2015090, projekt VaV |
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| MUNI/A/1116/2014, interní kód MU |
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| MUNI/11/InGA09/2012, interní kód MU |
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