FAFÍLEK, Bohumil, M KRAUSOVA, M VOJTECHOVA, Vendula POSPÍCHALOVÁ, L TUMOVA, E SLONCOVA, M HURANOVA, J STANCIKOVA, A HLAVATA, J SVEC, R SEDLACEK, O LUKSAN, M OLIVERIUS, Ivan VOSKA, Milan JIRSA, J PACES, M KOLAR, M KRIVJANSKA, K KLIMESOVA, H TLASKALOVA-HOGENOVA a V KORINEK. Troy, a Tumor Necrosis Factor Receptor Family Member, Interacts With Lgr5 to Inhibit Wnt Signaling in Intestinal Stem Cells. Gastroenterology. Philadelphia: W B Saunders co-Elsevier Inc, 2013, roč. 144, č. 2, s. 381-391. ISSN 0016-5085. Dostupné z: https://dx.doi.org/10.1053/j.gastro.2012.10.048. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1316069, author = {Fafílek, Bohumil and Krausova, M and Vojtechova, M and Pospíchalová, Vendula and Tumova, L and Sloncova, E and Huranova, M and Stancikova, J and Hlavata, A and Svec, J and Sedlacek, R and Luksan, O and Oliverius, M and Voska, Ivan and Jirsa, Milan and Paces, J and Kolar, M and Krivjanska, M and Klimesova, K and TlaskalovaandHogenova, H and Korinek, V}, article_location = {Philadelphia}, article_number = {2}, doi = {http://dx.doi.org/10.1053/j.gastro.2012.10.048}, keywords = {Mouse Model of Colon Cancer; beta-Catenin; TCF; Tnfrsf19}, language = {eng}, issn = {0016-5085}, journal = {Gastroenterology}, title = {Troy, a Tumor Necrosis Factor Receptor Family Member, Interacts With Lgr5 to Inhibit Wnt Signaling in Intestinal Stem Cells}, volume = {144}, year = {2013} }
TY - JOUR ID - 1316069 AU - Fafílek, Bohumil - Krausova, M - Vojtechova, M - Pospíchalová, Vendula - Tumova, L - Sloncova, E - Huranova, M - Stancikova, J - Hlavata, A - Svec, J - Sedlacek, R - Luksan, O - Oliverius, M - Voska, Ivan - Jirsa, Milan - Paces, J - Kolar, M - Krivjanska, M - Klimesova, K - Tlaskalova-Hogenova, H - Korinek, V PY - 2013 TI - Troy, a Tumor Necrosis Factor Receptor Family Member, Interacts With Lgr5 to Inhibit Wnt Signaling in Intestinal Stem Cells JF - Gastroenterology VL - 144 IS - 2 SP - 381-391 EP - 381-391 PB - W B Saunders co-Elsevier Inc SN - 00165085 KW - Mouse Model of Colon Cancer KW - beta-Catenin KW - TCF KW - Tnfrsf19 N2 - BACKGROUND & AIMS: The Wnt signaling pathway is required for maintenance of the intestinal epithelia; blocking this pathway reduces the proliferative capacity of the intestinal stem cells. However, aberrant Wnt signaling leads to intestinal cancer. We investigated the roles of the Wnt pathway in homeostasis of the intestinal epithelium and during malignant transformation in human cells and mice. METHODS: We performed chromatin immunoprecipitation (ChIP) with DNA microarray analysis (ChIP-on-chip) to identify genes regulated by Wnt signaling in human colorectal cancer cells Colo320, DLD1, LS174T, and SW480. Formation of intestinal tumor was induced in C57BL/6J mice using azoxymethane and dextran sulfate. Intestinal tissues from these mice, as well as Apc(+/Min) and Apc(CKO/CKO)/Lgr5-EGFP-IRES-CreERT2 mice, were analyzed by immunohistochemistry and in situ hybridization. RESULTS: We identified promoter regions of 960 genes that interacted with the Wnt pathway nuclear effector T-cell factor 4 in 4 different human colorectal cancer-derived cell lines; 18 of these promoters were present in all chromatin precipitates. Wnt signaling up-regulated a member of the tumor necrosis factor receptor superfamily called TROY. Levels of TROY messenger RNA were increased in human cells with deficiencies in the adenomatous polyposis coli (APC) gene and in cells stimulated with the Wnt3a ligand. Expression of Troy was significantly up-regulated in neoplastic tissues from mice during intestinal tumorigenesis. Lineage tracing experiments revealed that Troy is produced specifically by fast-cycling intestinal stem cells. TROY associated with a unique marker of these cells, leucine-rich repeat-containing G-protein coupled receptor (LGR) 5. In organoids established from the intestinal crypts, Troy suppressed signaling mediated by R-spondin, a Wnt agonist. CONCLUSIONS: TROY is up-regulated in human colorectal cancer cell lines and in intestinal tumors in mice. It functions as a negative modulator of the Wnt pathway in LGR5-positive stem cells. ER -
FAFÍLEK, Bohumil, M KRAUSOVA, M VOJTECHOVA, Vendula POSPÍCHALOVÁ, L TUMOVA, E SLONCOVA, M HURANOVA, J STANCIKOVA, A HLAVATA, J SVEC, R SEDLACEK, O LUKSAN, M OLIVERIUS, Ivan VOSKA, Milan JIRSA, J PACES, M KOLAR, M KRIVJANSKA, K KLIMESOVA, H TLASKALOVA-HOGENOVA a V KORINEK. Troy, a Tumor Necrosis Factor Receptor Family Member, Interacts With Lgr5 to Inhibit Wnt Signaling in Intestinal Stem Cells. \textit{Gastroenterology}. Philadelphia: W B Saunders co-Elsevier Inc, 2013, roč.~144, č.~2, s.~381-391. ISSN~0016-5085. Dostupné z: https://dx.doi.org/10.1053/j.gastro.2012.10.048.
|