| KOLLAR, Peter, Tomáš BÁRTA, Stanislava KELTOŠOVÁ, Pavlína TRNOVÁ, Veronika ZÁVALOVÁ MÜLLER, Karel ŠMEJKAL, Jan HOŠEK, Radek FEDR, Karel SOUČEK a Aleš HAMPL. Flavonoid 4 '-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, 2015, roč. 2015, "neuvedeno", s. 1-8. ISSN 1741-427X. Dostupné z: https://dx.doi.org/10.1155/2015/251895. |
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@article{1317741,
author = {Kollar, Peter and Bárta, Tomáš and Keltošová, Stanislava and Trnová, Pavlína and Závalová Müller, Veronika and Šmejkal, Karel and Hošek, Jan and Fedr, Radek and Souček, Karel and Hampl, Aleš},
article_location = {New York},
article_number = {"neuvedeno"},
doi = {http://dx.doi.org/10.1155/2015/251895},
keywords = {ACTIVATED PROTEIN-KINASE; RETINOIDS; INHIBITION; PATHWAYS},
language = {eng},
issn = {1741-427X},
journal = {Evidence-based Complementary and Alternative Medicine},
title = {Flavonoid 4 '-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells},
volume = {2015},
year = {2015}
}
TY - JOUR
ID - 1317741
AU - Kollar, Peter - Bárta, Tomáš - Keltošová, Stanislava - Trnová, Pavlína - Závalová Müller, Veronika - Šmejkal, Karel - Hošek, Jan - Fedr, Radek - Souček, Karel - Hampl, Aleš
PY - 2015
TI - Flavonoid 4 '-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells
JF - Evidence-based Complementary and Alternative Medicine
VL - 2015
IS - "neuvedeno"
SP - 1-8
EP - 1-8
PB - Hindawi Publishing Corporation
SN - 1741427X
KW - ACTIVATED PROTEIN-KINASE
KW - RETINOIDS
KW - INHIBITION
KW - PATHWAYS
N2 - Aims. In this work we studied cytodifferentiation effects of newly characterized prenyl flavonoid 4'-O-methylkuwanon E (4ME) isolated from white mulberry (Morus alba L.). Main Methods. Cell growth and viability were measured by dye exclusion assay; cell cycle and surface antigen CD11b were monitored by flow cytometry. For the cytodifferentiation of cells the NBT reduction assay was employed. Regulatory proteins were assessed by western blotting. Key Findings. 4ME induced dose-dependent growth inhibition of THP-1 cells, which was not accompanied by toxic effect. Inhibition of cells proliferation caused by 4ME was associated with the accumulation in G1 phase and with downregulation of hyperphosphorylated pRb. Treatment with 4ME led to significant induction of NBT-reducing activity of PMA stimulated THP-1 cells and upregulation expression of differentiation-associated surface antigen CD11b. Our results suggest that monocytic differentiation induced by 4ME is connected with up-regulation of p38 kinase activity. Significance. Our study provides the first evidence that 4ME induces the differentiation of THP-1 human monocytic leukemia cells and thus is a potential cytodifferentiating anticancer agent.
ER -
KOLLAR, Peter, Tomáš BÁRTA, Stanislava KELTOŠOVÁ, Pavlína TRNOVÁ, Veronika ZÁVALOVÁ MÜLLER, Karel ŠMEJKAL, Jan HOŠEK, Radek FEDR, Karel SOUČEK a Aleš HAMPL. Flavonoid 4 '-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. \textit{Evidence-based Complementary and Alternative Medicine}. New York: Hindawi Publishing Corporation, 2015, roč.~2015, ''neuvedeno'', s.~1-8. ISSN~1741-427X. Dostupné z: https://dx.doi.org/10.1155/2015/251895.
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