| BÁRTA, Tomáš, Lucie PEŠKOVÁ, Joseph COLLIN, David MONTANER, Irina NEGANOVA, Lyle ARMSTRONG and Majlinda LAKO. Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells. Stem Cells. Hoboken: Wiley-Blackwell, 2016, vol. 34, No 1, p. 246-251. ISSN 1066-5099. Available from: https://dx.doi.org/10.1002/stem.2220. |
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@article{1317743,
author = {Bárta, Tomáš and Pešková, Lucie and Collin, Joseph and Montaner, David and Neganova, Irina and Armstrong, Lyle and Lako, Majlinda},
article_location = {Hoboken},
article_number = {1},
doi = {http://dx.doi.org/10.1002/stem.2220},
keywords = {microRNA; miR-145; Induced pluripotent stem cells; Reprogramming; Mesenchymal-to-epithelial transition; OCT4; SOX2; KLF4; c-MYC},
language = {eng},
issn = {1066-5099},
journal = {Stem Cells},
title = {Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells},
volume = {34},
year = {2016}
}
TY - JOUR
ID - 1317743
AU - Bárta, Tomáš - Pešková, Lucie - Collin, Joseph - Montaner, David - Neganova, Irina - Armstrong, Lyle - Lako, Majlinda
PY - 2016
TI - Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells
JF - Stem Cells
VL - 34
IS - 1
SP - 246-251
EP - 246-251
PB - Wiley-Blackwell
SN - 10665099
KW - microRNA
KW - miR-145
KW - Induced pluripotent stem cells
KW - Reprogramming
KW - Mesenchymal-to-epithelial transition
KW - OCT4
KW - SOX2
KW - KLF4
KW - c-MYC
N2 - MicroRNA (miRNAs) are short noncoding RNA molecules involved in many cellular processes and shown to play a key role in somatic cell induced reprogramming. We performed an array based screening to identify candidates that are differentially expressed between dermal skin fibroblasts (DFs) and induced pluripotent stem cells (iPSCs). We focused our investigations on miR-145 and showed that this candidate is highly expressed in DFs relative to iPSCs and significantly downregulated during reprogramming process. Inhibition of miR-145 in DFs led to the induction of “cellular plasticity” demonstrated by: (a) alteration of cell morphology associated with downregulation of mesenchymal and upregulation of epithelial markers; (b) upregulation of pluripotency-associated genes including SOX2, KLF4, C-MYC; (c) downregulation of miRNA let-7b known to inhibit reprogramming; and (iv) increased efficiency of reprogramming to iPSCs in the presence of reprogramming factors. Together, our results indicate a direct functional link between miR-145 and molecular pathways underlying reprogramming of somatic cells to iPSCs.
ER -
BÁRTA, Tomáš, Lucie PEŠKOVÁ, Joseph COLLIN, David MONTANER, Irina NEGANOVA, Lyle ARMSTRONG and Majlinda LAKO. Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, 2016, vol.~34, No~1, p.~246-251. ISSN~1066-5099. Available from: https://dx.doi.org/10.1002/stem.2220.
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