| SLANINOVÁ, Iva, Noelia LÓPEZ-SÁNCHEZ, Kristýna ŠEBRLOVÁ, Ondřej VYMAZAL, José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. Biology of the Cell. Hoboken: Wiley-Blackwell, 2016, vol. 108, No 1, p. 1-18. ISSN 0248-4900. Available from: https://dx.doi.org/10.1111/boc.201500047. |
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@article{1318390,
author = {Slaninová, Iva and LópezandSánchez, Noelia and Šebrlová, Kristýna and Vymazal, Ondřej and Frade, José María and Táborská, Eva},
article_location = {Hoboken},
article_number = {1},
doi = {http://dx.doi.org/10.1111/boc.201500047},
keywords = {Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging},
language = {eng},
issn = {0248-4900},
journal = {Biology of the Cell},
title = {Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting},
volume = {108},
year = {2016}
}
TY - JOUR
ID - 1318390
AU - Slaninová, Iva - López-Sánchez, Noelia - Šebrlová, Kristýna - Vymazal, Ondřej - Frade, José María - Táborská, Eva
PY - 2016
TI - Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
JF - Biology of the Cell
VL - 108
IS - 1
SP - 1-18
EP - 1-18
PB - Wiley-Blackwell
SN - 02484900
KW - Cell cycle
KW - DNA dye
KW - FACS
KW - Macarpine
KW - Time-lapse imaging
N2 - Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.
ER -
SLANINOVÁ, Iva, Noelia LÓPEZ-SÁNCHEZ, Kristýna ŠEBRLOVÁ, Ondřej VYMAZAL, José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. \textit{Biology of the Cell}. Hoboken: Wiley-Blackwell, 2016, vol.~108, No~1, p.~1-18. ISSN~0248-4900. Available from: https://dx.doi.org/10.1111/boc.201500047.
|
